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Titolo:
Keratinocyte growth factor expression in human gingival fibroblasts and stimulation of in vitro gene expression by retinoic acid
Autore:
Mackenzie, IC; Gao, ZR;
Indirizzi:
Univ Wales Coll Med, Dept Adult Dent Hlth, Cardiff CF4 4XN, S Glam, Wales Univ Wales Coll Med Cardiff S Glam Wales CF4 4XN f CF4 4XN, S Glam, Wales
Titolo Testata:
JOURNAL OF PERIODONTOLOGY
fascicolo: 4, volume: 72, anno: 2001,
pagine: 445 - 453
SICI:
0022-3492(200104)72:4<445:KGFEIH>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
STROMAL-EPITHELIAL INTERACTIONS; IN-VITRO; DIFFERENTIATION; MORPHOGENESIS; INDUCTION; CYTOKINES; RECEPTORS; FAMILY; WOUNDS; CELLS;
Keywords:
epithelial attachment; epithelium; fibroblasts; gingiva; growth factors, keratinocyte; wound healing; growth factors, fibroblast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Mackenzie, IC Univ Wales Coll Med, Dept Adult Dent Hlth, Heath Pk, CardiffCF4 4XN, S Glam, Wales Univ Wales Coll Med Heath Pk Cardiff S Glam Wales CF4 4XN es
Citazione:
I.C. Mackenzie e Z.R. Gao, "Keratinocyte growth factor expression in human gingival fibroblasts and stimulation of in vitro gene expression by retinoic acid", J PERIODONT, 72(4), 2001, pp. 445-453

Abstract

Background: Keratinocyte growth factor (KGF) is a stromally derived growthfactor of the fibroblast growth factor (FGF) family with paracrine effectstargeted to influence the growth and differentiation of epithelia. Regional and temporal changes in KGF expression play important roles in the development and maintenance of epithelial structures and in epithelial wound healing. Differing patterns of expression of KGF by fibroblasts in the gingivalregion could therefore be related to the observed regional variation in the differentiation and behavior of gingival epithelia. Methods: The in vitro and in vivo patterns of expression of KGF mRNA in human gingival and periodontal fibroblasts were examined using reverse transcription polymerase chain reactions (RT-PCR) and in situ hybridization with digoxigenin-labeled riboprobes. The patterns observed for human gingiva were compared with those for human skin and for murine tissues. Results: Gingival and periodontal fibroblasts showed expression of KGF transcripts in vitro, and the degree of expression was markedly influenced by the presence of retinoic acid, an agent known to influence patterns of epithelial differentiation. Sections of human and murine gingiva and skin showed regionally variable expression of transcripts with the cells expressing KGF in the subepithelial, rather than the deeper, connective tissues and periodontium. Conclusions: The results point to a role of KGF in the maintenance of normal growth and differentiation of gingival epithelia. A lack of KGF expression by periodontal fibroblasts in vivo is expected to hinder apical epithelial migration and thus stabilize the epithelial attachment. The effects of retinoic acid (RA) on KGF expression in vitro provide an indirect mechanism by which RA may regulate the growth and differentiation of gingival epithelia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:19:49