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Titolo:
Effects of protein tyrosine kinase inhibitor genistein on retinal functionin superfused vertebrate retina
Autore:
Luke, C; Krott, R; Luke, M; Lebek, J; Walter, P; Brunner, R; Sickel, W;
Indirizzi:
Univ Cologne, Zentrum Augenheilkunde, Dept Ophthalmol, D-50924 Cologne, Germany Univ Cologne Cologne Germany D-50924 phthalmol, D-50924 Cologne, Germany
Titolo Testata:
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
fascicolo: 2, volume: 17, anno: 2001,
pagine: 151 - 158
SICI:
1080-7683(200104)17:2<151:EOPTKI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; ISCHEMIA-REPERFUSION INJURY; TOPOISOMERASE-II; NEOVASCULARIZATION; CELLS; ANGIOGENESIS; RETINOPATHY; SUPPRESSION; INDUCTION; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Luke, C Univ Cologne, Zentrum Augenheilkunde, Dept Ophthalmol, Joseph Stelzmann Str 9, D-50924 Cologne, Germany Univ Cologne Joseph Stelzmann Str 9 Cologne Germany D-50924 rmany
Citazione:
C. Luke et al., "Effects of protein tyrosine kinase inhibitor genistein on retinal functionin superfused vertebrate retina", J OCUL PH T, 17(2), 2001, pp. 151-158

Abstract

The aim of this study was to evaluate safe concentrations of genistein fora potential intraocular application using the isolated retina technique onbovine retina preparations. Bovine retinas were isolated and perfused with an oxygen pre-equilibrated standard solution. The L electroretinogram (ERG) was recorded as a transretinal potential using silver/silver-chloride electrodes. After recording of stable ERG amplitudes, genistein was added to the solution in different concentrations. The percentage of b-wave reduction under the drug was calculated. We also studied the influence of genistein on the a-wave amplitude. After the addition of aspartate, the b-wave amplitude was reduced continuouslyuntil unmasked a-wave amplitudes were reached. Genistein was then added tothe aspartate containing perfusate. The percentage of a-wave amplitude reduction under the drug was calculated. Concentrations of 3.3 mu Mol/l and higher were found to reduce the b-wave amplitude. The a-wave amplitude was not changed by the applied concentrations. The ERG only showed toxic effects fi-om genistein beyond concentrationsthat were found to inhibit endothelial cell growth in vitro. In previous studies, beneficial effects on trabecular mesh-work cells were present For genistein concentrations which are distinctly higher than the maximum nontoxic concentration reported here. It was shown that the photoreceptor layer is not affected at the examined concentration range. Therefore, we attributethe toxic effects to postsynaptic interaction of genistein. Intraocular application of genistein in a sufficient concentration seems possible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:02:19