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Titolo:
Excitatory nicotinic and desensitizing muscarinic (M2) effects on C-nociceptors in isolated rat skin
Autore:
Bernardini, N; Sauer, SK; Haberberger, R; Fischer, MJM; Reeh, PW;
Indirizzi:
Univ Erlangen Nurnberg, Inst Physiol & Expt Pathophysiol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Giessen, Inst Anat & Zellbiol, D-353885 Giessen, Germany Univ Giessen Giessen Germany D-353885 ellbiol, D-353885 Giessen, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 21, anno: 2001,
pagine: 3295 - 3302
SICI:
0270-6474(20010501)21:9<3295:ENADM(>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSAL-ROOT GANGLIA; RECEPTOR MESSENGER-RNA; ACETYLCHOLINE-RECEPTORS; SENSORY NEURONS; IN-VITRO; BIOLOGICAL-SYSTEMS; EXPRESSION; SUBTYPES; ANTINOCICEPTION; CLASSIFICATION;
Keywords:
acetylcholine; analgesia; cholinergic; mechanosensitivity; pain; sensitization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Bernardini, N Univ Erlangen Nurnberg, Inst Physiol & Expt Pathophysiol, Univ Str 17, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Univ Str 17 Erlangen Germany D-91054
Citazione:
N. Bernardini et al., "Excitatory nicotinic and desensitizing muscarinic (M2) effects on C-nociceptors in isolated rat skin", J NEUROSC, 21(9), 2001, pp. 3295-3302

Abstract

The actions of different cholinergic agonists and antagonists were investigated on nociceptive afferents using the rat skin-saphenous nerve preparation, in vitro. Nicotine was able to weakly excite C-nociceptors and to induce a mild sensitization to heat stimulation (in 77% of tested fibers) in a dose-dependent manner (10(-6) to 10(-5) M), but it caused no alteration in mechanical responsiveness tested with von Frey hairs. Muscarine did not induce a significant nociceptor excitation, but almost all fibers exhibited a marked desensitization to mechanical and heat stimuli in a dose-dependent manner (from 10(-6) to 10(-5) M). The muscarinic effects could be prevented by the general muscarinic antagonist scopolamine (10(-5) M), by the M3 antagonist 1,1-dimethyl-4-diphenylacetoxypiperidium oxide (10(-6) M) co-applied with the M2 antagonist gallamine (10(-5) M), and by gallamine alone. As positive control we used the relatively M2-selective agonist arecaidine (10(-6) to 10(-5) M), obtaining a similar desensitizing effect as with muscarine. Finally, we performed an immunocytochemical study that demonstrated the presence of M2 but not M3 receptors in thin epidermal nerve fibers of the rathairy skin. Altogether, these data demonstrate opposite effects of nicotinic and muscarinic receptor stimulation on cutaneous nociceptors. M2 receptor-mediated depression of nociceptive responsiveness may convey a therapeutic, i.e., analgesic or antinociceptive, potential.

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Documento generato il 20/01/20 alle ore 04:52:37