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Titolo:
GABA(A) receptor alpha 1 subunit deletion prevents developmental changes of inhibitory synaptic currents in cerebellar neurons
Autore:
Vicini, S; Ferguson, C; Prybylowski, K; Kralic, J; Morrow, AL; Homanics, GE;
Indirizzi:
Georgetown Univ, Sch Med, Dept Physiol & Biophys, Washington, DC 20007 USAGeorgetown Univ Washington DC USA 20007 Biophys, Washington, DC 20007 USA Univ Pittsburgh, Dept Anesthesiol Crit Care Med, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 are Med, Pittsburgh, PA 15261 USA Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15261 USA Univ PittsburghPittsburgh PA USA 15261 armacol, Pittsburgh, PA 15261 USA Univ N Carolina, Sch Med, Dept Pharmacol & Psychiat, Chapel Hill, NC 27599USA Univ N Carolina Chapel Hill NC USA 27599 ychiat, Chapel Hill, NC 27599USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 21, anno: 2001,
pagine: 3009 - 3016
SICI:
0270-6474(20010501)21:9<3009:GRA1SD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONIC STEM-CELLS; GRANULE CELLS; A RECEPTORS; MESSENGER-RNAS; RAT-BRAIN; POSTNATAL-DEVELOPMENT; MINIATURE IPSCS; STELLATE CELLS; EXPRESSION; KINETICS;
Keywords:
GABA receptor; gene knock-out; patch-clamp; inhibitory synapses; development; GABA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Vicini, S Georgetown Univ, Sch Med, Dept Physiol & Biophys, 3900 ReservoirRd NW, Washington, DC 20007 USA Georgetown Univ 3900 Reservoir Rd NW Washington DC USA 20007 USA
Citazione:
S. Vicini et al., "GABA(A) receptor alpha 1 subunit deletion prevents developmental changes of inhibitory synaptic currents in cerebellar neurons", J NEUROSC, 21(9), 2001, pp. 3009-3016

Abstract

Developmental changes in miniature IPSC (mIPSC) kinetics have been demonstrated previously in cerebellar neurons in rodents. We report that these kinetic changes in mice are determined primarily by developmental changes in GABAA receptor subunit expression. mIPSCs were studied by whole-cell recordings in cerebellar slices, prepared from postnatal day 11 (P11) and P35 mice. Similar to reports in granule neurons, wild-type cerebellar stellate neuron mIPSCs at P11 had slow decay kinetics, whereas P35 mIPSCs decayed five times faster. When mIPSCs in cerebellar stellate neurons were compared between wild-type (+/+) and GABA(A) receptor alpha1 subunit-deficient (-/-) littermates at P35, we observed dramatically slower mIPSC decay rates in -/- animals. We took advantage of the greater potency of imidazopyridines for GABA current potentiation with alpha1 subunit-containing receptors to characterize the relative contribution of alpha1 subunits in native receptors on inhibitory synapses of cerebellar granule neurons. Zolpidem-induced prolongation of mIPSC decay was variable among distinct cells, but it increased during development in wild-type mice. Similarly, Zolpidem prolongation of mIPSCdecay rate was significantly greater in adult +/+ mice than in knock-outs. We propose that an increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents that are normally observed during postnatal development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:38:41