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Titolo:
delta- and gamma-sarcoglycan localization in the sarcoplasmic reticulum ofskeletal muscle
Autore:
Ueda, H; Ueda, K; Baba, T; Ohno, S;
Indirizzi:
Yamanashi Med Univ, Dept Anat, Tamaho, Yamanashi 4093898, Japan Yamanashi Med Univ Tamaho Yamanashi Japan 4093898 amanashi 4093898, Japan
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 4, volume: 49, anno: 2001,
pagine: 529 - 537
SICI:
0022-1554(200104)49:4<529:DAGLIT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYSTROPHIN-GLYCOPROTEIN COMPLEX; MUSCULAR-DYSTROPHY; ULTRASTRUCTURAL-LOCALIZATION; ALPHA-SARCOGLYCAN; BETA-SARCOGLYCAN; EXTRACELLULAR-MATRIX; EPSILON-SARCOGLYCAN; MYOTONIC-DYSTROPHY; DEFICIENT MICE; PROTEIN-KINASE;
Keywords:
sarcoglycan; sarcoplasmic reticulum; terminal cisternae; skeletal muscle; dystrophin complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ueda, H Yamanashi Med Univ, Dept Anat, Tamaho, Yamanashi 4093898, Japan Yamanashi Med Univ Tamaho Yamanashi Japan 4093898 4093898, Japan
Citazione:
H. Ueda et al., "delta- and gamma-sarcoglycan localization in the sarcoplasmic reticulum ofskeletal muscle", J HIST CYTO, 49(4), 2001, pp. 529-537

Abstract

Sarcoglycans are transmembrane proteins that are members of the dystrophincomplex. Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle fibers. However, it is still unclear whether or not sarcoglycans are restricted to thesarcolemma. To address this issue, we examined alpha-, beta-, delta-, and gamma -sarcoglycan expression in femoral skeletal muscle from control and dystrophin-deficient mice and rats using confocal microscopy and immunoelectron microscopy. Confocal microscopy of the tissues in cross-section showed that all sarcoglycans were detected under the sarcolemma in rats and control mice. delta- and gamma -sarcoglycan labeling demonstrated striations in the longitudinal section, suggesting that the proteins were expressed in thesarcoplasmic reticulum (SR) or transverse tubules (T-tubules). Moreover, such striations of both sarcoglycans were recognized in the dystrophin-deficient mouse skeletal muscle. Double labeling with phalloidin or alpha -actinin and delta- or gamma -sarcoglycan showed different labeling patterns, indicating that delta -sarcoglycan localization was distinct from that of gamma -sarcoglycan. Immunoelectron microscopy clarified that delta -sarcoglycanwas localized in the terminal cisternae of the SR, while gamma -sarcoglycan was found in the terminal cisternae and longitudinal SR over I-bands but not over A-bands. These data demonstrate that delta- and gamma -sarcoglycans are components of the SR in skeletal muscle, suggesting that both sarcoglycans function independent of the dystrophin complex in the SR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:58:19