Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Opposing actions of nitric oxide on synaptic inputs of identified interneurones in the central nervous system of the crayfish
Autore:
Aonuma, H; Newland, PL;
Indirizzi:
Univ Southampton, Sch Biol Sci, Div Cell Sci, Southampton SO16 7PX, Hants,England Univ Southampton Southampton Hants England SO16 7PX 16 7PX, Hants,England
Titolo Testata:
JOURNAL OF EXPERIMENTAL BIOLOGY
fascicolo: 7, volume: 204, anno: 2001,
pagine: 1319 - 1332
SICI:
0022-0949(200104)204:7<1319:OAONOO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TERMINAL ABDOMINAL-GANGLION; NADPH-DIAPHORASE HISTOCHEMISTRY; NMDA RECEPTOR ACTIVATION; ASCENDING INTERNEURONS; PRESYNAPTIC INHIBITION; GUANYLATE-CYCLASE; PROCAMBARUS-CLARKII; THORACIC GANGLIA; NEURAL NETWORKS; MUSHROOM BODIES;
Keywords:
NO-cGMP signalling pathway; neuromodulation; ascending interneurone; sensory neurone; local circuit; crayfish; Pacifastacus leniusculus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Aonuma, H Hokkaido Univ, Res Inst Elect Sci, Lab Neurocybernet, Sapporo, Hokkaido 0600812, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600812 ido 0600812, Japan
Citazione:
H. Aonuma e P.L. Newland, "Opposing actions of nitric oxide on synaptic inputs of identified interneurones in the central nervous system of the crayfish", J EXP BIOL, 204(7), 2001, pp. 1319-1332

Abstract

Little is known of the action of nitric oxide (NO) at the synaptic level on identified interneurones in local circuits that process mechanosensory signals. Here, we examine the action of NO in the terminal abdominal ganglionof the crayfish Pacifastacus leniusculus, where it has modulatory effects on the synaptic inputs of 17 identified ascending interneurones mediated byelectrical stimulation of a sensory nerve. To analyse the role of NO in the processing of sensory signals, we bath-applied the NO donor SNAP, the NO scavenger PTIO, the nitric oxide synthase (NOS) inhibitor L-NAME, the NOS substrate L-arginine, a cyclic GMP (cGMP) analogue, 8-Br-cGMP, and the soluble guanylate cyclase (sGC) inhibitor ODQ. The effects of these chemicals onthe synaptic inputs of the interneurones could be divided into two distinct classes. The NO donor SNAP enhanced the inputs to one class of interneurone (class 1) and depressed those to another (class 2). Neither the inactiveisomer NAP nor degassed SNAP had any effect on the inputs to these same classes of interneurone, The NO scavenger PTIO caused the opposite effects tothose of the NO donor SNAP, indicating that endogenous NO may have an action in local circuits. Preventing the synthesis of NO using L-NAME had the opposite effect to that of SNAP an each response class of interneurone, Increasing the synthesis of endogenous NO by applying L-arginine Led to effectson both response classes of interneurone similar to those of SNAP, Taken together, these results suggested that NO was the active component in mediating the changes in amplitude of the excitatory postsynaptic potentials. Finally, the effects of 8-Br-cGMP were similar to those of the NO donor, indicating the possible involvement of a NO-sensitive guanylate cyclase, This was confirmed by preventing the synthesis of cGMP by sGC using ODQ, which caused the opposite effects to those of 8-Br-cGMP on the two response classes of interneurone, The results indicate that a NO-cGMP signal transduction pathway, in which NO regulates transmitter release from mechanosensory afferents onto intersegmental ascending interneurones, is probably present in thelocal circuits of the crayfish.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:59:53