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Titolo:
Induction of a non-rhythmic motor pattern by nitric oxide in hatchling Rana temporaria embryos
Autore:
McLean, DL; McDearmid, JR; Sillar, KT;
Indirizzi:
Univ St Andrews, Sch Biol, Div Biomed Sci, St Andrews KY16 9TS, Fife, Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS Y16 9TS, Fife, Scotland CUNY Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA CUNYMt Sinai Sch Med New York NY USA 10029 ophys, New York, NY 10029 USA
Titolo Testata:
JOURNAL OF EXPERIMENTAL BIOLOGY
fascicolo: 7, volume: 204, anno: 2001,
pagine: 1307 - 1317
SICI:
0022-0949(200104)204:7<1307:IOANMP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS-LAEVIS EMBRYOS; CENTRAL-NERVOUS-SYSTEM; NEONATAL RAT; SPINAL NEURONS; PINEAL EYE; MODULATION; RESPONSES; BEHAVIOR; SYNTHASE; CORD;
Keywords:
nitric oxide; frog; Rana temporaria; motor pattern; swimming; NADPH diaphorase; histochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: McLean, DL Univ St Andrews, Sch Biol, Div Biomed Sci, Bute Med Bldg, St Andrews KY16 9TS, Fife, Scotland Univ St Andrews Bute Med Bldg St Andrews Fife Scotland KY16 9TS
Citazione:
D.L. McLean et al., "Induction of a non-rhythmic motor pattern by nitric oxide in hatchling Rana temporaria embryos", J EXP BIOL, 204(7), 2001, pp. 1307-1317

Abstract

Nitric oxide (NO) is a ubiquitous neuromodulator with a diverse array of functions in a variety of brain regions, but a role for NO in the generationof locomotor activity has yet to be demonstrated. The possibility that NO is involved in the generation of motor activity in embryos of the frog Ranatemporaria was investigated using the NO donors S-nitroso-n-acetylpenicillamine (SNAP; 100-500 mu mol l(-1)) and diethylamine nitric oxide complex sodium (DEANO; 25-100 mu mol l(-1)). Immobilised Rana temporaria embryos generate a non-rhythmic 'lashing' motor pattern either spontaneously or in response to dimming of the experimental bath illumination, Path-applied NO donors triggered a qualitatively similar motor pattern in which non-rhythmic motor bursts were generated contra- and ipsilaterally down the length of the body. The inactive precursor of SNAP, n-acetyl-penicillamine (NAP), at equivalent concentrations did not trigger motor activity. NO donors failed to initiate swimming and had no measurable effects on the parameters of swimming induced by electrical stimulation. Intracellular recordings with potassium-acetate-filled electrodes revealed that the bursts of ventral root discharge induced by NO donors were accompanied by phasic depolarisations in motor neurons, During the inter-burst intervals, periods of substantial membrane hyperpolarisation below the normal resting potential were observed, presumably coincident with contralateral ventral root activity, With KCl-filled electrodes, inhibitory potentials were strongly depolarising, suggesting that inhibition was Cl--dependent, The synaptic drive seen in motor neurons after dimming of the illumination was very similar to that induced by the NOdonors. NADPH-diaphorase histochemistry identified putative endogenous sources of NO in the central nervous system and the skin. Three populations ofbilaterally symmetrical neurons mere identified within the brainstem, Someof these neurons had contralateral projections and many had axonal processes that projected to and entered the marginal zones of the spinal cord, suggesting that they were reticulospinal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:13:14