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Titolo:
FUNGAL AND BACTERIAL DISEASE RESISTANCE IN TRANSGENIC PLANTS EXPRESSING HUMAN LYSOZYME
Autore:
NAKAJIMA H; MURANAKA T; ISHIGE F; AKUTSU K; OEDA K;
Indirizzi:
SUMITOMO CHEM CO LTD,BIOTECHNOL LAB,4 CHOME TAKARAZUKA HYOGO 665 JAPAN SUMITOMO CHEM CO LTD,BIOTECHNOL LAB TAKARAZUKA HYOGO 665 JAPAN IBARAKI UNIV,FAC AGR AMI IBARAKI 3003 JAPAN
Titolo Testata:
Plant cell reports
fascicolo: 10, volume: 16, anno: 1997,
pagine: 674 - 679
SICI:
0721-7714(1997)16:10<674:FABDRI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOBACCO; PROTEINS;
Keywords:
HUMAN LYSOZYME; TRANSGENIC TOBACCO PLANTS; DISEASE RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
H. Nakajima et al., "FUNGAL AND BACTERIAL DISEASE RESISTANCE IN TRANSGENIC PLANTS EXPRESSING HUMAN LYSOZYME", Plant cell reports, 16(10), 1997, pp. 674-679

Abstract

The human lysozyme gene, which is assembled by the stepwise ligation of chemically synthesized oligonucleotides, was introduced into tobacco (Nicotiana tabacum cv 'SR1') by the Agrobacterium-mediated method. The introduced human lysozyme gene was highly expressed under the control of the cauliflower mosaic virus 35S promoter, and the gene product accumulated in the transgenic tobacco plants. The transgenic tobacco plants showed enhanced resistance against the fungus Erysiphe cichoracearum - both conidia formation and mycelial growth were reduced, and the size of the colony was diminished. Microscopic observation revealed that the transgenic tobacco plants carried the resistant phenotype, analogous to that of the resistant cultivar 'Kokubu' which had been selected by conventional breeding. Growth of the phytopathogenic bacteriumPseudomonas syringae pv. tabaci was also strongly retarded in the transgenic tobacco, and the chlorotic halo of the disease symptom was reduced to 17% of that observed in the wild-type tobacco. Thus, the introduction of a human lysozyme gene is an effective approach to crops against both fungal and bacterial diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:39:42