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Titolo:
Sensitive liquid chromatographic-tandem mass spectrometric method for the determination of fluoxetine and its primary active metabolite norfluoxetinein human plasma
Autore:
Sutherland, FCW; Badenhorst, D; de Jager, AD; Scanes, T; Hundt, HKL; Swart, KJ; Hundt, AF;
Indirizzi:
Univ Orange Free State, FARMOVS Res Ctr Clin Pharmacol & Drug Dev, ZA-9300Bloemfontein, South Africa Univ Orange Free State Bloemfontein South Africa ZA-9300 n, South Africa
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 914, anno: 2001,
pagine: 45 - 51
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION; ANTIDEPRESSANTS;
Keywords:
kinetic studies; validation; pharmaceutical analysis; fluoxetine; norfluoxetine; doxepin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Sutherland, FCW Univ Orange Free State, FARMOVS Res Ctr Clin Pharmacol & Drug Dev, POB 339,G6, ZA-9300 Bloemfontein, South Africa Univ Orange Free State POB 339,G6 Bloemfontein South Africa ZA-9300
Citazione:
F.C.W. Sutherland et al., "Sensitive liquid chromatographic-tandem mass spectrometric method for the determination of fluoxetine and its primary active metabolite norfluoxetinein human plasma", J CHROMAT A, 914(1-2), 2001, pp. 45-51

Abstract

A sensitive method for the simultaneous determination of fluoxetine and its major active metabolite norfluoxetine in plasma was developed, using high-performance liquid chromatographic separation with tandem mass spectrometric detection. The samples were extracted from alkalised plasma with hexane-isoamyl alcohol (98.2, v/v) followed by back-extraction into formic acid (2%). Chromatography was performed on a Phenomenex (R) Luna C-18 (2) 5 mum, 150X2 mm column with a mobile phase consisting of acetonitrile-0.02% formic acid (340:660, v/v) at a flow-rate of 0.35 ml/min. Detection was achieved by a Perkin-Elmer Sciex API 2000 mass spectrometer (LC-MS-MS) set at unit resolution in the multiple reaction monitoring mode. TurboIonSpray ionisationwas used for ion production. The mean recoveries for fluoxetine and norfluoxetine were 98 and 97%, respectively, with a lower limit of quantificationset at 0.15 ng/ml for the analyte and its metabolite. This assay method makes use of the increased sensitivity and selectivity of mass spectrometric (MS-MS) detection to allow for a more rapid (extraction and chromatography)and sensitive method for the simultaneous determination of fluoxetine and norfluoxetine in human plasma than has previously been described. (C) 2001 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 10:09:10