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Titolo:
ADAM8: A novel osteoclast stimulating factor
Autore:
Choi, SJ; Han, JH; Roodman, GD;
Indirizzi:
Audie L Murphy Mem Vet Hosp, San Antonio, TX 78284 USA Audie L Murphy Mem Vet Hosp San Antonio TX USA 78284 ntonio, TX 78284 USA Univ Texas, Hlth Sci Ctr, Dept Med Hematol, San Antonio, TX USA Univ Texas San Antonio TX USA Ctr, Dept Med Hematol, San Antonio, TX USA
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 5, volume: 16, anno: 2001,
pagine: 814 - 822
SICI:
0884-0431(200105)16:5<814:AANOSF>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE T-ANTIGEN; BCL-X-L; BONE-RESORPTION; METALLOPROTEASE DOMAIN; MEMBRANE-PROTEINS; DISINTEGRIN; FAMILY; CELL; GENE; IDENTIFICATION;
Keywords:
ADAM8; macrophage colony-stimulating factor; polymerase chain reaction; osteoclasts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Roodman, GD Audie L Murphy Mem Vet Hosp, 7400 Merton Minter Blvd, San Antonio, TX 78284 USA Audie L Murphy Mem Vet Hosp 7400 Merton Minter Blvd San Antonio TX USA 78284
Citazione:
S.J. Choi et al., "ADAM8: A novel osteoclast stimulating factor", J BONE MIN, 16(5), 2001, pp. 814-822

Abstract

We used polymerase chain reaction (PCR)-selective complementary DNA (cDNA)subtraction hybridization with an immortalized murine osteoclast (OCL) precursor cell line to identify genes that are highly expressed in OCLs compared with OCL precursors and which map be involved in the OCL differentiationprocess. ADAM8 was one of the 50 genes identified. ADAM (a disintegrin andmetalloproteinase) peptides are membrane-bound proteins that can act as cell-to-cell and cell-to-matrix adhesion molecules, degrade the extracellularmatrix, and play a role in tissue morphogenesis, Addition of antisense (AS) S-oligonucleotides for ADAM8 (1-10 nM) to mouse bone marrow cultures treated with 10(-9) Ri 1,25-dihgdroxyvitamin D-3 [1,25(OH)(2)D-3] significantlyinhibited OCL formation compared with treatment with the control S-oligonucleotide, Furthermore, conditioned media from 293 cells transiently transfected with a secretable form of the ADAM8 cDNA increased OCL formation in a dose-dependent manner. In addition, treatment of OCLs with soluble ADAM8 conditioned media significantly increased pit formation per dentin slice compared with control OCLs. Time course studies indicated that ADAM8 increased OCL formation only when it was present during days 4-7 of the 7-day cultureperiod. Structural analysis, using truncated constructs of ADAM8, showed that the cysteine-rich/disintegrin domain was responsible for its OCL stimulatory activity, Western blot analysis confirmed that the soluble form of ADAM8 is present in normal marrow cultures, These data suggest that ADAM8 plays an important role in OCL formation and acts primarily at the later stages of OCL differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:54:36