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Titolo:
Characterization of a novel isoform of capase-9 that inhibits apoptosis
Autore:
Angelastro, JM; Moon, NY; Liu, DX; Yang, AS; Greene, LA; Franke, TF;
Indirizzi:
Columbia Univ, Coll Phys & Surg, Dept Pathol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 , Dept Pathol, New York, NY 10032 USA Columbia Univ, Coll Phys & Surg, Ctr Neurobiol & Behav, New York, NY 10032USA Columbia Univ New York NY USA 10032 robiol & Behav, New York, NY 10032USA Columbia Univ, Coll Phys & Surg, Dept Pharmacol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ept Pharmacol, New York, NY 10032 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 15, volume: 276, anno: 2001,
pagine: 12190 - 12200
SICI:
0021-9258(20010413)276:15<12190:COANIO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEATH; CASPASE-9; CELLS; IDENTIFICATION; PROCASPASE-9; ACTIVATION; NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Angelastro, JM Columbia Univ, Coll Phys & Surg, Dept Pathol, 630 W 168th St,P&S15-401, New York, NY 10032 USA Columbia Univ 630 W 168th St,P&S15-401 New York NY USA 10032
Citazione:
J.M. Angelastro et al., "Characterization of a novel isoform of capase-9 that inhibits apoptosis", J BIOL CHEM, 276(15), 2001, pp. 12190-12200

Abstract

We have identified a novel isoform of rat caspase-9 in which the C terminus of full-length caspase-9 is replaced with an alternative peptide sequence. Casp-9-CTD (where CTD is carboxyl-terminal divergent) is expressed in multiple tissues, with the relative highest expression observed in ovary and heart. Casp-9-CTD was found primarily in the cytoplasm and was not detected in the nucleus. Structural predictions suggest that in contrast to full-length caspase-9, casp-9-CTD will not be processed. Our model is supported by reduced protease activity of casp-9-CTD preparations in vitro and by the lack of detectable processing of casp-9-CTD proenzyme or the induction of cell death following transfection into cells. Both neuronal and non-neuronal cell types transfected with casp-9-CTD were resistant to death evoked by trophic factor deprivation or DNA damage. In addition, cytosolic lysates prepared from cells permanently expressing exogenous casp-9-CTD were resistant to caspase induction by cytochrome c in reconstitution assays. Taken together, our observations indicate that casp-9-CTD acts as a dominant-negative variant. Its expression in various tissues indicates a physiological role in regulating cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:35:02