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Titolo:
Two new elastin cross-links having pyridine skeleton - Implication of ammonia in elastin cross-linking in vivo
Autore:
Umeda, H; Takeuchi, M; Suyama, K;
Indirizzi:
Tohoku Univ, Dept Appl Bioorgan Chem, Div Life Sci, Grad Sch Agr Sci,Aoba Ku, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Ku, Sendai, Miyagi 9818555, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 16, volume: 276, anno: 2001,
pagine: 12579 - 12587
SICI:
0021-9258(20010420)276:16<12579:TNECHP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; AMINO-ACIDS; AGE; AORTA; COLLAGEN; RAT; MATURATION; CIRRHOSIS; ALKANALS; TISSUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Suyama, K Tohoku Univ, Dept Appl Bioorgan Chem, Div Life Sci, Grad Sch AgrSci,Aoba Ku, 1-1 Tsutsumidori Amamiya, Sendai, Miyagi 9818555, Japan Tohoku Univ 1-1 Tsutsumidori Amamiya Sendai Miyagi Japan 9818555
Citazione:
H. Umeda et al., "Two new elastin cross-links having pyridine skeleton - Implication of ammonia in elastin cross-linking in vivo", J BIOL CHEM, 276(16), 2001, pp. 12579-12587

Abstract

Isolation and structure analysis of two amino acids from bovine ligamentumnuchae elastin hydrolysates revealed the presence of pyridine cross-links in elastin. The structures of these amino acids were determined to have 3,4,5- and 2,3,5-trisubstituted pyridine skeletons both with three carboxylic acids and a mass of 396 (C18H28N4O6) identified as 4-(4-amino-4-carboxybutyl)3,5-di-(3-amino-3-carboxypropyl)-pyridine and 2-(4-amino-4-carboxybutyl)-3,5-di-(3-amino-3-carboxypropyl)-pyridine, We have named these pyridine cross-links desmopyridine (DESP) and isodesmopyridine (IDP), respectively. Structure analysis of these pyridine crosslinks implied that the formation of these cross-links involved the condensation reaction between ammonia and allysine. The elastin incubated with ammonium chloride showed that DESP and IDP levels increased as the allysine content decreased. DESP and IDP were measured by high pressure liquid chromatography (HPLC) with W detection and were found in a variety of bovine tissues. The DESP/desmosine (DES) and IDP/isodesmosine (IDE) ratios in aorta elastin were higher than in other tissues. DESP and IDP contents in human aorta elastin were found to be gradually increased with age. The concentration of IDP was significantly elevated in aorta elastin of rat with chronic liver cirrhosis induced by carbon tetrachloride (mean +/- S.D.; 11.1 +/- 0.9 nmol/mg elastin) when compared with normal rats (5.9 +/- 1.5 nmol/mg elastin), Although DESP and IDP are present at only trace concentrations in the tissue elastin, these pyridine cross-links may be useful biomarkers for the aortic elastin damaged by ammonia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:12:34