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Titolo:
Involvement of the pro-oncoprotein TLS (translocated in liposarcoma) in nuclear factor-kappa B p65-mediated transcription as a coactivator
Autore:
Uranishi, H; Tetsuka, T; Yamashita, M; Asamitsu, K; Shimizu, M; Itoh, M; Okamoto, T;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Mol Genet, Mizuho Ku, Aichi 4678601, JapanNagoya City Univ Aichi Japan 4678601 et, Mizuho Ku, Aichi 4678601, Japan Nagoya City Univ, Sch Med, Dept Internal Med 1, Mizuho Ku, Aichi 4678601, Japan Nagoya City Univ Aichi Japan 4678601 1, Mizuho Ku, Aichi 4678601, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 16, volume: 276, anno: 2001,
pagine: 13395 - 13401
SICI:
0021-9258(20010420)276:16<13395:IOTPT(>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TATA-BINDING PROTEIN; RNA-POLYMERASE-II; THYROID-HORMONE RECEPTORS; HUMAN MYELOID-LEUKEMIA; TUMOR-NECROSIS-FACTOR; RAT MESANGIAL CELLS; DNA-BINDING; CHROMOSOMAL TRANSLOCATION; ACTIVATION DOMAIN; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Okamoto, T Nagoya City Univ, Sch Med, Dept Mol Genet, Mizuho Ku, 1 Kawasumi, Aichi 4678601, Japan Nagoya City Univ 1 Kawasumi Aichi Japan 4678601 4678601, Japan
Citazione:
H. Uranishi et al., "Involvement of the pro-oncoprotein TLS (translocated in liposarcoma) in nuclear factor-kappa B p65-mediated transcription as a coactivator", J BIOL CHEM, 276(16), 2001, pp. 13395-13401

Abstract

In this study, we have demonstrated that translocated in liposarcoma (TLS), also termed FUS, is an interacting molecule of the p65 (Re1A) subunit of the transcription factor nuclear factor kappaB (NF-kappaB) using a yeast two-hybrid screen. We confirmed the interaction between TLS and p65 by the pull-down assay in vitro and by a coimmunoprecipitation experiment followed by Western blot of the cultured cell in vivo, TLS was originally identified as part of a fusion protein with CHOP arising from chromosomal translocation in human myxoid liposarcomas. TLS has been shown to be involved in TFIID complex formation and associated with RNA polymerase II. However, the role of TLS in transcriptional regulation has not yet been clearly elucidated. We found that TLS en hanced the NF-kappaB-mediated transactivation induced by physiological stimuli such as tumor necrosis factor alpha, interleukin-1 beta, and overexpression of NF-kappaB-inducing kinase, TLS augmented NF-kappaB-dependent promoter activity of the intercellular adhesion molecule-1 gene and interferon-beta gene. These results suggest that TLS acts as a coactivator of NF-kappaB and plays a pivotal role in the NP-kappaB-mediated transactivation.

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Documento generato il 19/09/20 alle ore 23:25:31