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Titolo:
Plant regeneration from protoplasts of Enteromorpha intestinalis (Chlorophyta, Ulvophyceae) as seedstock for macroagal culture
Autore:
Rusig, AM; Cosson, J;
Indirizzi:
Univ Caen, F-14032 Caen, France Univ Caen Caen France F-14032Univ Caen, F-14032 Caen, France
Titolo Testata:
JOURNAL OF APPLIED PHYCOLOGY
fascicolo: 2, volume: 13, anno: 2001,
pagine: 103 - 108
SICI:
0921-8971(200104)13:2<103:PRFPOE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALGAL SEED STOCK; SEAWEEDS; ENZYMES;
Keywords:
protoplasts; Enteromorpha intestinalis; regeneration; aquaculture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Rusig, AM Univ Caen, BP 5186, F-14032 Caen, France Univ Caen BP 5186 CaenFrance F-14032 186, F-14032 Caen, France
Citazione:
A.M. Rusig e J. Cosson, "Plant regeneration from protoplasts of Enteromorpha intestinalis (Chlorophyta, Ulvophyceae) as seedstock for macroagal culture", J APPL PHYC, 13(2), 2001, pp. 103-108

Abstract

A continuous micropropagation was established from protoplasts of the green alga Enteromorpha intestinalis. The effects of two different crude enzymes and the osmolarity at different concentrations of the enzyme solution on algal protoplast yields were tested. The optimal enzyme composition for cell wall digestion and protoplast viability was 2% cellulase R 10 Onozuka and2% Aplysie with 0.5 m mannitol. Large numbers of Enteromorpha protoplasts were released (10.0 x 10(6) protoplasts from 1 g fresh thalli) and settled on a range of substrata. Regeneration of the protoplasts followed the normal pattern for this species. Conditions for pure cultures and efficient systems of floating supports with nets were determined to optimise the product quality of plantlets of Enteromorpha. A promising storage process has been developed which involves including protoplasts in beads of alginic acid gel. Plants regenerated from protoplasts may also be used as seedstock to facilitate propagation for macroalgal culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 11:33:47