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Titolo:
Cytoskeletal-induced alterations in the adhesion of HT-1080 fibrosarcoma cells to extracellular matrix
Autore:
Murakami, M; Dirks, PB; Hubbard, SL; Rutka, JT;
Indirizzi:
Univ Toronto, Hosp Sick Children, Brain Tumor Res Lab, Toronto, ON M5G 1X8, Canada Univ Toronto Toronto ON Canada M5G 1X8 s Lab, Toronto, ON M5G 1X8, Canada
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 5, volume: 18, anno: 2001,
pagine: 1027 - 1033
SICI:
1019-6439(200105)18:5<1027:CAITAO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; IV COLLAGEN; GENE-EXPRESSION; MONOCLONAL-ANTIBODY; NEURITE OUTGROWTH; SYNTHETIC PEPTIDE; MELANOMA-CELLS; LAMININ; FIBRONECTIN; MIGRATION;
Keywords:
cytoskeleton; glial fibrillary acidic protein; fibrosarcoma; adhesion; extracellular matrix;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Murakami, M Kumamoto Univ, Sch Med, Dept Neurosurg, 1-1-1 Honjo Kumamoto, Kumamoto 860, Japan Kumamoto Univ 1-1-1 Honjo Kumamoto Kumamoto Japan 860 , Japan
Citazione:
M. Murakami et al., "Cytoskeletal-induced alterations in the adhesion of HT-1080 fibrosarcoma cells to extracellular matrix", INT J ONCOL, 18(5), 2001, pp. 1027-1033

Abstract

We have investigated the adhesion of the human fibrosarcoma cell line, HT-1080, transfected with glial fibrillary acidic protein (GFAP) to a variety of extracellular matrix macromolecules (ECM) including collagen type IV, laminin, and fibronectin. The GFAP-transfectants demonstrated altered adhesiveness to extracellular matrix substrates when compared to controls. GFAP-positive, heavy metal-induced fibrosarcoma cells were more adherent to plastic and collagen type IV than were the parental or uninduced cells. In contrast, GFAP-positive fibrosarcoma cells were less adherent to laminin- or fibronectin-coated dishes than controls. Time course adhesion studies over 9 days showed that the heavy metal-induced fibrosarcoma cells progressively became more adherent to collagen type IV and less adherent to laminin- or fibronectin-coated dishes than did uninduced cells. However, with the removal of heavy metal from the medium, the HT-1080 fibrosarcoma cells were restoredto their original adhesive potential. By phase microscopy, uninduced and induced MT-1080 cells demonstrated different morphological features and remained viable in an anchorage-dependent fashion on collagen type IV as a substrate. By way of contrast, GFAP-induced MT-1080 cells were not particularlyviable in monolayer culture and readily detached from laminin as a substrate. The expression of beta1 integrin in GFAP-positive fibrosarcoma cells was decreased following heavy metal induction by Western blot analyses. In contrast, the expression of alpha2 integrin was increased whereas alpha5 integrin was unchanged in MT-1080 cells following the induction of GFAP. Gelatin zymography showed that 72 kDa collagenase was less expressed in GFAP-induced clones than in controls. Our data suggest that the forced expression ofthe intermediate filament, GFAP, in MT-1080 cells may modulate cell adhesion to different ECM substrates through alterations in expression of integrins.

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Documento generato il 28/03/20 alle ore 09:28:05