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Titolo:
Induction of RANTES chemokine expression in human astrocytic cells is dependent upon activation of NF-kappa B transcription factor
Autore:
Li, QDQ; Bever, CT; Burt, DR; Judge, SIV; Trisler, GD;
Indirizzi:
Univ Maryland, Sch Med, Dept Pharmacol & Expt Therapeut, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 t Therapeut, Baltimore, MD 21201 USA Univ Maryland, Sch Med, VA Maryland Hlth Care Syst, Med Res & Neurol Serv,Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Neurol Serv,Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Neurol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Dept Neurol, Baltimore, MD 21201 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 5, volume: 7, anno: 2001,
pagine: 527 - 533
SICI:
1107-3756(200105)7:5<527:IORCEI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; TRANSFORMING GROWTH FACTOR-BETA-1; MULTIPLE-SCLEROSIS LESIONS; MESSENGER-RNA EXPRESSION; GENE-EXPRESSION; AUTOIMMUNE ENCEPHALOMYELITIS; ALPHA PROTEOLYSIS; ENDOTHELIAL-CELLS; CYTOKINE RANTES; RAT ASTROCYTES;
Keywords:
TNF-alpha; RANTES; chemokine; astrocyte; glia; NF-kappa B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Li, QDQ 7806 Hvenside Terrace, Rockville, MD 20855 USA 7806 Hvenside Terrace Rockville MD USA 20855 kville, MD 20855 USA
Citazione:
Q.D.Q. Li et al., "Induction of RANTES chemokine expression in human astrocytic cells is dependent upon activation of NF-kappa B transcription factor", INT J MOL M, 7(5), 2001, pp. 527-533

Abstract

RANTES is a C-C (beta)-family chemokine that is implicated in the migration of peripheral blood leukocytes to brain lesions in multiple sclerosis (MS), an inflammatory demyelinating disease of the central nervous system (CNS). Glial cells are active participants in the inflammatory response in the CNS, and they have been shown to respond to and produce a number of cytokines and chemokines in vivo and in vitro. Recently, we have shown inducibility of RANTES gene expression by TNF-alpha in human astrocytic cells. Therefore, the goal of the current study was to investigate the transcription activating factor involved in the process. We found that the induction of RANTES mRNA and protein by TNF-alpha in human astrocytic cells is associated with increased NF-kappaB DNA-binding activity. p65 and p50 were determined to be the components of the activated NF-kappaB transcription factor complex by supershift assay. In addition, the blockade of NF-kappaB activation by three known NF-kappaB inhibitors markedly reduced the TNF-alpha -induced RANTES expression at the mRNA and protein levels. Furthermore, the reduction inNF-kappaB binding activity to the promoter of the human RANTES gene causedby the NF-kappaB inhibitors parallels a decrease in RANTES expression in these cells. Our data suggest that NF-kappaB may mediate the induction of RANTES gene expression, in human glial cells, through its cognate cis-acting element.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:50:18