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Titolo:
Upregulation of toll-like receptor 2 gene expression in macrophage response to peptidoglycan and high concentration of lipopolysaccharide is involvedin NF-kappa B activation
Autore:
Liu, Y; Wang, Y; Yamakuchi, M; Isowaki, S; Nagata, E; Kanmura, Y; Kitajima, I; Maruyama, I;
Indirizzi:
Kagoshima Univ, Sch Med, Dept Lab & Mol Med, Kagoshima 8908520, Japan Kagoshima Univ Kagoshima Japan 8908520 Mol Med, Kagoshima 8908520, Japan Kagoshima Univ, Fac Med, Dept Anesthesiol & Crit Care Med, Kagoshima 8908520, Japan Kagoshima Univ Kagoshima Japan 8908520 are Med, Kagoshima 8908520, Japan
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 5, volume: 69, anno: 2001,
pagine: 2788 - 2796
SICI:
0019-9567(200105)69:5<2788:UOTR2G>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-WALL COMPONENTS; INDUCED SIGNAL-TRANSDUCTION; DERMAL ENDOTHELIAL-CELLS; CUTTING EDGE; BACTERIAL LIPOPOLYSACCHARIDE; DROSOPHILA TOLL; DIFFERENTIAL EXPRESSION; TRANSCRIPTION FACTORS; LIPOTEICHOIC ACID; MOUSE MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Maruyama, I Kagoshima Univ, Sch Med, Dept Lab & Mol Med, 8-35-1 Sakuragaoka, Kagoshima8908520, Japan Kagoshima Univ 8-35-1 Sakuragaoka Kagoshima Japan 8908520 pan
Citazione:
Y. Liu et al., "Upregulation of toll-like receptor 2 gene expression in macrophage response to peptidoglycan and high concentration of lipopolysaccharide is involvedin NF-kappa B activation", INFEC IMMUN, 69(5), 2001, pp. 2788-2796

Abstract

Toll-like receptors 2 and 4 (TLR2 and TLR4) hare been found to transduce signals of peptidoglycan (PGN) and lipopolysaccharide (LPS), respectively, for NF-kappaB activation. However, little is known about the expression and regulation of the TLR2 gene in monocytes/macrophages in response to the twotypical bacterial products. We show in the present study that both PGN anda high concentration of LPS increase TLR2 gene expression in macrophage-like cells, 1 alpha ,25-dihydrovitamin D-3-differentiated human HL60 and mouse RAW264.7 cells, and human monocytes in a dose- and time-dependent manner. Actinomycin D and pyrrolidine dithiocarbamate inhibition of gene transcription and NF-kappaB activation, respectively, blocks LPS- and PGN-elevated TLR2 mRNA in monocytic cells, The LPS-induced increase in TLR2 mRNA in monocytic cells is abolished bq polymyxin B pretreatment and is observed in peripheral blood mononuclear cells from pigs subjected to endotoxic shock. Further, high concentrations of LPS and synthetic lipid A increase TLR2 mRNA expression in peritoneal macrophages from both TLR4-deficient C3H/HeJ mice and normal C3H/HeN mice, a process that constitutes induction of TLR4-independent TLR2 expression, These findings demonstrate that TLR2 gene expression is upregulated in macrophage responses to PGN and to high concentrations ofLPS in vitro and in vivo and correlates with NF-KB activation.

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Documento generato il 03/07/20 alle ore 16:46:20