Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Qualitative and quantitative analysts of monocyte transendothelial migration by confocal microscopy and three-dimensional image reconstruction
Autore:
Andjeklovic, AV; Zochowski, MR; Morgan, F; Pachter, JS;
Indirizzi:
Univ Connecticut, Ctr Hlth, Dept Pharmacol, Blood Brain Barrier Lab, Farmington, CT 06030 USA Univ Connecticut Farmington CT USA 06030 er Lab, Farmington, CT 06030 USA Univ Connecticut, Ctr Hlth, Ctr Biomed Imaging, Farmington, CT 06030 USA Univ Connecticut Farmington CT USA 06030 maging, Farmington, CT 06030 USA Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 r & Mol Physiol, New Haven, CT 06510 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 2, volume: 37, anno: 2001,
pagine: 111 - 120
SICI:
1071-2690(200102)37:2<111:QAQAOM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; CENTRAL-NERVOUS-SYSTEM; ENDOTHELIAL-CELLS; IN-VITRO; BINDING-SITES; EXPRESSION; CHEMOKINES; TRANSMIGRATION; MONOLAYERS; ADHESION;
Keywords:
endothelial cells; leukocytes; extravasation; blood-brain barrier;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Pachter, JS Univ Connecticut, Ctr Hlth, Dept Pharmacol, Blood Brain Barrier Lab, 263 Farmington Ave, Farmington, CT 06030 USA Univ Connecticut 263 Farmington Ave Farmington CT USA 06030 SA
Citazione:
A.V. Andjeklovic et al., "Qualitative and quantitative analysts of monocyte transendothelial migration by confocal microscopy and three-dimensional image reconstruction", IN VITRO-AN, 37(2), 2001, pp. 111-120

Abstract

A novel for qualitative and quantitative analysis of monocyte transendothelial migration is described. By labeling monocytes and endothelial cells with different fluorophores, and utilizing confocal microscopy and three-dimensional image reconstruction, transmigrating monocytes were resolved and quantified within a subendothelial collagen gel. Comparison of monocyte migration across endothelial monolayers derived from human brain microvessels versus umbilical veins revealed diapedesis across brain endothelium to be significantly delayed. Inclusion of astrocytes within the sub-endothelial collagen gel resulted in the formation of an array of astrocytic processes thatsimulated the glia limitans surrounding brain microvessels in situ, thus yielding a more physiologic paradigm of the blood-brain barrier. By virtue of its unique capacity to provide information on the total number of migrating cells, this analytic approach overcomes significant caveats associated with sampling only aspects of the migration process. The potential adaptability of this method to computer-assisted analysis further enhances its prospective use in high-throughput screening.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:28:54