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Titolo:
Erythroid cell growth and differentiation in vitro in the simulated microgravity environment of the NASA rotating wall vessel bioreactor
Autore:
Sytkowski, AJ; Davis, KL;
Indirizzi:
Harvard Univ, Sch Med, Dept Med,Beth Israel Deaconess Med Ctr, Div Hematol& Oncol,Lab Cell & Mol Biol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ab Cell & Mol Biol, Boston, MA 02115 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 2, volume: 37, anno: 2001,
pagine: 79 - 83
SICI:
1071-2690(200102)37:2<79:ECGADI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; RED-BLOOD-CELLS; MEDIATED ENDOTHELIAL MECHANOTRANSDUCTION; 3-DIMENSIONAL CULTURE; SIGNAL-TRANSDUCTION; SPACE-FLIGHT; ERYTHROLEUKEMIA-CELLS; HUMAN-LYMPHOCYTES; SHEAR-STRESS; ERYTHROPOIETIN;
Keywords:
erythropoiesis; microgravity; erythropoietin; anemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Sytkowski, AJ Beth Israel Deaconess Med Ctr, 330 Brookline Ave W-BL 548, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr 330 Brookline Ave W-BL 548 Boston MA USA 02215
Citazione:
A.J. Sytkowski e K.L. Davis, "Erythroid cell growth and differentiation in vitro in the simulated microgravity environment of the NASA rotating wall vessel bioreactor", IN VITRO-AN, 37(2), 2001, pp. 79-83

Abstract

Prolonged exposure of humans and experimental animals to the altered gravitational conditions of space flight has adverse effects on the lymphoid anderythroid hematopoietic systems. Although some information is available regarding the cellular and molecular changes in lymphocytes exposed to microgravity, little is known about the erythroid cellular changes that may underlie the reduction in erythropoiesis and resultant anemia. We now report a reduction in erythroid growth and a profound inhibition of erythropoietin (Epo)-induced differentiation in a ground-based simulated microgravity model system. Rauscher murine erythroleukemia cells were grown either in tissue culture vessels at 1 x g or in the simulated microgravity environment of theNASA-designed rotating wall vessel (RWV) bioreactor. Logarithmic growth was observed under both conditions; however, the doubling time in simulated microgravity was only one-half of that seen at 1 x g. No difference in apoptosis was detected. Induction with Epo at the initiation of the culture resulted in differentiation of approximately 25% of the cells at 1 x g, consistent with our previous observations. In contrast, induction with Epo at the initiation of simulated microgravity resulted in only one-half of this degree of differentiation. Significantly, the growth of cells in simulated microgravity for 24 h prior to Epo induction inhibited the differentiation almost completely. The results suggest that the NASA RWV bioreactor may serve as a suitable ground-based microgravity simulator to model the cellular and molecular changes in erythroid cells observed in true microgravity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:55:00