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Titolo:
Proliferation of human hematopoietic bone marrow cells in simulated microgravity
Autore:
Plett, RA; Frankovitz, SM; Abonour, R; Orschell-Traycoff, CM;
Indirizzi:
Indiana Univ, Sch Med, Dept Med, Div Hematol Oncol, Indianapolis, IN 46202USA Indiana Univ Indianapolis IN USA 46202 l Oncol, Indianapolis, IN 46202USA Indiana Univ, Sch Med, Dept Med, Indiana Elks Canc Res Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 es Ctr, Indianapolis, IN 46202 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 2, volume: 37, anno: 2001,
pagine: 73 - 78
SICI:
1071-2690(200102)37:2<73:POHHBM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; MURINE STROMAL CELLS; EX-VIVO EXPANSION; PROGENITOR CELLS; CD34(+) CELLS; SPACE-FLIGHT; BLOOD; ACTIVATION; INVITRO; DIFFERENTIATION;
Keywords:
bone marrow; stem cells; CD34(+); cell culture; cell cycle; ex vivo expansion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Plett, RA Indiana Univ, Sch Med, Dept Med, Div Hematol Oncol, 1044 W Walnut St,R4-202, Indianapolis, IN 46202 USA Indiana Univ 1044 W Walnut St,R4-202 Indianapolis IN USA 46202 A
Citazione:
R.A. Plett et al., "Proliferation of human hematopoietic bone marrow cells in simulated microgravity", IN VITRO-AN, 37(2), 2001, pp. 73-78

Abstract

Expansion and/or maintenance of hematopoietic stem cell (HSC) potential following in vitro culture remains a major obstacle in stem cell biology and bone marrow (BM) transplantation. Several studies suggest that culture of mammalian cells in microgravity (mu -g) may reduce proliferation and differentiation of these cells. We investigated the application of these findings to the field of stem cell biology in the hopes of expanding HSC with minimal loss of hematopoietic function. To this end, BM CD34(+) cells were cultured for 4-6 d in rotating wall vessels for simulation of mu -g, and assessedfor expansion, cell cycle activation, apoptosis, and hematopoietic potential. While CD34(+) cells cultured in normal gravity (1-g) proliferated up tothreefold by day 4-6, cells cultured in mu -g did not increase in number. As a possible explanation for this, cells cultured in simulated mu -g were found to exit G(0)/G(1), phase of cell cycle at a slower rate than 1-g controls. When assayed for primitive hematopoietic potential in secondary conventional 1-g long-term cultures, cells from initial mu -g cultures produced greater numbers of cells and progenitors, and for a Longer period of time, than cultures initiated with 1-g control cells. Similar low levels of apoptosis and adhesion molecule phenotype in mu -g and 1-g-cultured cells suggested similar growth patterns in the two settings. These data begin to elucidate the effects of mu -g on proliferation of human hematopoietic cells and may he potentially beneficial to the fields of stem cell biology and somatic gene therapy.

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Documento generato il 09/04/20 alle ore 09:22:09