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Titolo:
Molecular cloning of the orphanin FQ receptor gene and differential tissueexpression of splice variants in rat
Autore:
Curro, D; Yoo, JH; Anderson, M; Song, I; Del Valle, J; Owyang, C;
Indirizzi:
Univ Michigan, Dept Internal Med, Gastrointestinal Paptide Ctr, Hlth Syst,Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 r, Hlth Syst,Ann Arbor, MI 48109 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 266, anno: 2001,
pagine: 139 - 145
SICI:
0378-1119(20010321)266:1-2<139:MCOTOF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
KAPPA(3)-RELATED OPIOID RECEPTOR; PROTEIN-COUPLED RECEPTOR; CDNA CLONING; MOUSE-BRAIN; FAMILY; MEMBER; FQ/NOCICEPTIN; LOCALIZATION; NEUROPEPTIDE; ANALGESIA;
Keywords:
exon/intron; transcription initiation sites; reverse transcription-polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Owyang, C Univ Michigan, Dept Internal Med, Gastrointestinal Paptide Ctr, Hlth Syst,Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 st,Ann Arbor, MI 48109 USA
Citazione:
D. Curro et al., "Molecular cloning of the orphanin FQ receptor gene and differential tissueexpression of splice variants in rat", GENE, 266(1-2), 2001, pp. 139-145

Abstract

Pharmacological and receptor-ligand binding studies of the cloned orphaninFQ (OFQ) receptor suggest that multiple forms of this receptor may exist. To further characterize the OFQ receptor (OFQR), we attempted to isolate the gene encoding this receptor in rat. The OFQR gene exceeds 10 kb in lengthand contains six exons ranging from 34 to 524 bp that are interrupted by five introns. The ATG translation initiation codon is located in exon, and the open reading frame consists of 1283 bp. Primer extension analysis of thegene revealed two major transcription initiation sites: one in the 5 ' Ranking region and the other in intron 1. The rat OFQR gene appeared to be alternatively spliced to yield multiple mRNAs. Four splice variants deleted for exon 1 were expressed only in brain. In contrast, five isoforms containing exon 1 were expressed in various tissues, such as brain, testes, and gastrointestinal tract. These data suggest that unique regions in intron 1 and in the 5 ' flanking region of the OFQR gene contribute to the regulation ofits expression in different tissues. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:25:37