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Titolo:
Genomic structure and chromosomal mapping of the gene coding for ICBP90, aprotein involved in the regulation of the topoisomerase II alpha gene expression
Autore:
Hopfner, R; Mousli, M; Garnier, JM; Redon, R; du Manoir, S; Chatton, B; Ghyselinck, N; Oudet, P; Bronner, C;
Indirizzi:
Univ Strasbourg 1, INSERM, CNRS, Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch, France Univ Strasbourg 1 Illkirch France F-67404 aire, F-67404 Illkirch, France Univ Louis Pasteur Strasbourg 1, INSERM U425, Fac Pharm, F-67401 Illkirch Graffenstaden, France Univ Louis Pasteur Strasbourg 1 Illkirch Graffenstaden France F-67401 ce
Titolo Testata:
GENE
fascicolo: 1-2, volume: 266, anno: 2001,
pagine: 15 - 23
SICI:
0378-1119(20010321)266:1-2<15:GSACMO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CANCER; CCAAT BOXES; PROMOTER; REGION; TRANSCRIPTION; ORGANIZATION; LEUKEMIA; 19P13.3; BINDING; CELLS;
Keywords:
cancer; cell proliferation; gene expression; ICBP90;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Bronner, C Univ Strasbourg 1, INSERM, CNRS, Inst Genet & Biol Mol & Cellulaire, BP 163, F-67404 Illkirch, France Univ Strasbourg 1 BP 163 Illkirch France F-67404 kirch, France
Citazione:
R. Hopfner et al., "Genomic structure and chromosomal mapping of the gene coding for ICBP90, aprotein involved in the regulation of the topoisomerase II alpha gene expression", GENE, 266(1-2), 2001, pp. 15-23

Abstract

We have recently identified a novel CCAAT box binding protein (ICBP90) involved in the regulation of topoisomerase II alpha gene expression. We have observed that it is expressed in non-tumoral proliferating human lung fibroblast cells whereas in HeLa cells, a tumoral cell line, ICBP90 was still present even when cells were at confluence. In the present study, we have determined the ICBP90 gene structure by screening of a human placenta genomic library and PCR analysis. We report that the ICBP90 gene spans about 35.8 kb and contains six coding exons named A to F. In the 5 ' upstream sequence of the region containing the coding exons. two additional exons (I and II) were found. Additionally, an internal splicing site was found in exon A. A promoter region, including three putative Spl binding sites between exons Iand A, was identified by transient transfection. Northern blot analysis ofseveral cancer cell lines revealed the existence of two ICBP90 mRNA species of 5.1 and 4.3 kb that are transcribed from the gene. The relative amounts of these mRNAs depended on the cell type. in MOLT-4 cells and Burkitts lymphoma Raji cells, the 4.3 kb or the 5.1 kb transcripts were mainly observed, respectively. In other cell lines, such as HL-60 cells, chronic myelogenous leukaemia K-562, lung carcinoma A549. HeLa or colorectal SW480, both 3.3 and 5.1 kb forms of ICBP90 mRNA could be detected, interestingly, westernblot analysis showed several ICBP90 protein bands in HeLa but only a single band in MOLT-4 cell extracts. Taken together our results are consistent with the ICBP90 gene exhibiting alternative splicing and promoter usage in acell-specific manner. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:18:22