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Titolo:
Protective effects of vitamins C and E on the number of micronuclei in lymphocytes in smokers and their role in ascorbate free radical formation in plasma
Autore:
Schneider, M; Diemer, K; Engelhart, K; Zankl, H; Tommer, WE; Biesalski, HK;
Indirizzi:
Univ Hohenheim, Inst Biol Chem & Ernaehrungswissensch, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 sch, D-70593 Stuttgart, Germany Univ Calif San Francisco, Gladstone Inst Cardiovasc Dis, Cardiovasc Res Inst, San Francisco, CA 94141 USA Univ Calif San Francisco San Francisco CA USA 94141 ancisco, CA 94141 USA Univ Kaiserslautern, Fachbereich Biol, Abt Humanbiol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern Kaiserslautern Germany D-67653 iserslautern, Germany Univ Kaiserslautern, Fachbereich Chem, Biochem Abt, D-67653 Kaiserslautern, Germany Univ Kaiserslautern Kaiserslautern Germany D-67653 iserslautern, Germany
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 3, volume: 34, anno: 2001,
pagine: 209 - 219
SICI:
1071-5762(2001)34:3<209:PEOVCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN-BLOOD-PLASMA; LIPID-PEROXIDATION; CIGARETTE-SMOKE; ALPHA-TOCOPHEROL; OXIDANTS; ANTIOXIDANTS; CHOLESTEROL; ATHEROSCLEROSIS; MORTALITY;
Keywords:
ascorbic acid; ascorbate free radical; alpha-tocopherol; EPR; micronuclei;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Biesalski, HK Univ Hohenheim, Inst Biol Chem & Ernaehrungswissensch, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 tuttgart, Germany
Citazione:
M. Schneider et al., "Protective effects of vitamins C and E on the number of micronuclei in lymphocytes in smokers and their role in ascorbate free radical formation in plasma", FREE RAD RE, 34(3), 2001, pp. 209-219

Abstract

Cigarette smoke is widely believed to increase free radical concentrationscausing subsequent oxidative processes that lead to DNA damage and hence, to several diseases including lung cancer and atheroselerosis. Vitamin C isa reducing agent that can terminate free-radical-driven oxidation by beingconverted to a resonance-stabilized free radical. To investigate whether short-term supplementation with the antioxidants vitamin C and E decreases free-radical-driven oxidation and thus decreases DNA damage in smokers, we determined the frequency of micronuclei in lymphocytes in 24 subjects and monitored the electron paramagnetic resonance signal of ascorbate free radical formation in plasma. Further parameters comprised sister-chromatid exchanges and thiobarbituric acid-reactive substances. Twelve smokers and twelve non-smokers took 1000 mg ascorbic acid daily for 7 days and then 1000 mg ascorbic acid and 335.5 mg RRR-alpha -tocopherol daily for the next 7 days. Baseline concentrations of both vitamins C and E were lower and baseline numbers of micronuclei were higher (p < 0.0001) in smokers than in non-smokers. After 7 days of vitamins C and E, DNA damage as monitored by the number of micronulei was decreased in both, smokers and non-smokers, but it was more decreased in smokers as indicated by fewer micronuclei in peripheral lymphocytes (p < 0.05). Concomitantly, the plasma concentrations of vitamin C (p < 0.001) as well as the ascorbate free radical (p < 0.05) were increased. The corresponding values in non-smokers, however, did not change. Our findings show that increased ascorbate free radical formation in plasma after short-term supplementation with vitamins C and E can decrease the number of micronuclei in blood lymphocytes and thus DNA damage in smokers.

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Documento generato il 24/01/20 alle ore 12:25:54