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Titolo:
Establishment of specific pathogen-free guinea-pig colonies using limited-flora guinea-pigs associated with conventional guinea-pig flora, and monitoring of their cecal flora
Autore:
Yanabe, M; Shibuya, M; Gonda, T; Asai, H; Tanaka, T; Sudou, K; Narita, T; Matsui, T; Itoh, K;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Lab Vet Publ Hlth, Bunkyo Ku, Tokyo 1338657, Japan Univ Tokyo Tokyo Japan 1338657 ubl Hlth, Bunkyo Ku, Tokyo 1338657, Japan Japan SLC Inc, Shizuoka 4338111, Japan Japan SLC Inc Shizuoka Japan 4338111 an SLC Inc, Shizuoka 4338111, Japan Univ Tokyo, Inst Med Sci, Tokyo 1080071, Japan Univ Tokyo Tokyo Japan 1080071 Tokyo, Inst Med Sci, Tokyo 1080071, Japan Sankyo Co Ltd, Shinagawa Ku, Tokyo 1400005, Japan Sankyo Co Ltd Tokyo Japan 1400005 td, Shinagawa Ku, Tokyo 1400005, Japan Obihiro Univ Agr & Vet Med, Dept Vet Pathol, Obihiro, Hokkaido 0808555, Japan Obihiro Univ Agr & Vet Med Obihiro Hokkaido Japan 0808555 0808555, Japan
Titolo Testata:
EXPERIMENTAL ANIMALS
fascicolo: 2, volume: 50, anno: 2001,
pagine: 105 - 113
SICI:
1341-1357(200104)50:2<105:EOSPGC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONIZATION RESISTANCE; GNOTOBIOTIC MICE; RABBITS;
Keywords:
cecal microflora; conventionalization; hysterectomy-derived animals; limited flora guinea-pigs; SPF guinea-pigs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Itoh, K Univ Tokyo, Grad Sch Agr & Life Sci, Lab Vet Publ Hlth, Bunkyo Ku,1-1-1 Yayoi, Tokyo 1338657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1338657 , Tokyo 1338657, Japan
Citazione:
M. Yanabe et al., "Establishment of specific pathogen-free guinea-pig colonies using limited-flora guinea-pigs associated with conventional guinea-pig flora, and monitoring of their cecal flora", EXP ANIM, 50(2), 2001, pp. 105-113

Abstract

Six groups of limited flora (LF) Hartley guinea-pigs were produced by inoculation of hysterectomy-derived GF guinea-pigs with various combinations ofcecal bacteria of conventional (CV) guinea-pigs to determine the effectivebacterial cocktails for the establishment of a specific pathogen free (SPF) colony. Bifidobacterium magnum (Bif) isolated from CV guinea-pigs was used for pretreatment. The mortality of LF guinea-pigs inoculated with only Bif was 75%, and that of those inoculated with Bif plus chloroform-treated cecal suspension (CHF) or Bif plus CHF plus 32 isolates from CV guinea-pigs was 40 to 66. 7%. These three groups were in an unhealthy condition with mucoid enteritis-like diarrhea. However, the mortality of LF guinea-pigs inoculated with the anaerobic growth on EG plates injected with 10(-5) dilution of cecal contents (CF) or inoculated with Bif plus CF was 6.3 and 15%, respectively. These latter two groups of LF guinea-pigs were transferred to separate barrier rooms and some of the LF guinea-pigs were maintained in isolators as a source of intestinal flora for SPF guinea-pigs. The composition of cecal flora of LF guinea-pigs was stable for a long time, and bacteroidaceae and peptococcaceae were maintained as predominant components. The basiccomposition of the cecal flora of SPF guinea-pigs originated from LF guinea-pigs, which consists mainly of the anaerobic bacteria, was not changed over a long period, and the flora composition became similar to that in CV guinea-pigs. Guinea-pig-specific pathogens from the SPF colonies were not defected during experiments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:11:30