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Titolo:
Inhibition of the myeloperoxidase chlorinating activity by non-steroidal anti-inflammatory drugs investigated with a human recombinant enzyme
Autore:
Neve, J; Parij, N; Moguilevsky, N;
Indirizzi:
Free Univ Brussels, Inst Pharm, Dept Pharmaceut Chem, B-1050 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1050 hem, B-1050 Brussels, Belgium Free Univ Brussels, Dept Appl Genet, B-6041 Gosselies, Belgium Free Univ Brussels Gosselies Belgium B-6041 t, B-6041 Gosselies, Belgium
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 1-2, volume: 417, anno: 2001,
pagine: 37 - 43
SICI:
0014-2999(20010406)417:1-2<37:IOTMCA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; HYPOCHLOROUS ACID PRODUCTION; HYDROGEN-PEROXIDE; HUMAN-NEUTROPHILS; LEUKOCYTE PEROXIDASES; OXIDATION; SUPEROXIDE; CHEMILUMINESCENCE; CHONDROCYTES; GENERATION;
Keywords:
NSAID (non-steroidal anti-inflammatory drug); myeloperoxidase; compound II; inflammation; recombinant human enzyme; reactive oxygen species;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Neve, J Free Univ Brussels, Inst Pharm, Dept Pharmaceut Chem, Campus Plaine 205-5,B-1050 Brussels, Belgium Free Univ Brussels Campus Plaine 205-5 Brussels Belgium B-1050 um
Citazione:
J. Neve et al., "Inhibition of the myeloperoxidase chlorinating activity by non-steroidal anti-inflammatory drugs investigated with a human recombinant enzyme", EUR J PHARM, 417(1-2), 2001, pp. 37-43

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) were investigated for theirability to affect the chlorinating activity of human myeloperoxidase and to scavenge HOCl, the main myeloperoxidase system product. Fourteen drugs representative of various NSAIDs families were tested with the chlorination of taurine used as a detection system. All were unable to inhibit taurine chlorination in a system without myeloperoxidase. In contrast, most of them induced a dose-dependent inhibition of the taurine chlorination mediated by a myeloperoxidase/H2O2/Cl- system. This took place at variable drug concentrations and TCS, were calculated. The inhibitory effect was therefore due to a direct interaction with the enzyme rather than to HOCl scavenging. A spectroscopic method used to measure the myeloperoxidase compound II lifetimein presence of the different drugs showed that all the drugs, which inhibited chlorination activity were able to induce accumulation of compound II. The extent of chlorinating activity inhibition (ICS,) was inversely relatedto the duration of the block of enzyme in compound II form. This further demonstrates that myeloperoxidase is an interesting target for anti-inflammatory therapy. The recombinant myeloperoxidase used for the first time in this kind of study was as convenient for pharmacological purposes as the purified one. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 31/03/20 alle ore 05:14:39