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Titolo:
Expression of immunoglobulin heavy chain transcripts (VH-families, IgM, and IgD) in head kidney and spleen of the Atlantic cod (Gadus morhua L.)
Autore:
Stenvik, J; Schroder, MB; Olsen, K; Zapata, A; Jorgensen, TO;
Indirizzi:
Univ Tromso, Dept Marine Biotechnol, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 Coll Fishery Sci, N-9037 Tromso, Norway Univ Complutense Madrid, Fac Ciencias Biol, Dept Biol Celular, Madrid, Spain Univ Complutense Madrid Madrid Spain , Dept Biol Celular, Madrid, Spain
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 4, volume: 25, anno: 2001,
pagine: 291 - 302
SICI:
0145-305X(200105)25:4<291:EOIHCT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMO-SALAR L; VARIABLE REGION DIVERSITY; CYPRINUS-CARPIO L; LIGHT-CHAIN; LYMPHOID ORGANS; MESSENGER-RNA; MONOCLONAL-ANTIBODIES; NUCLEOTIDE-SEQUENCES; INDUCED SUPPRESSION; POSITIVE CELLS;
Keywords:
Gadus morhua; immunoglobulin; heavy chain; IgM; IgD; VH-family; in situ hybridization; tyramide signal amplification; Northern blotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Stenvik, J Univ Tromso, Dept Marine Biotechnol, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 y Sci, N-9037 Tromso, Norway
Citazione:
J. Stenvik et al., "Expression of immunoglobulin heavy chain transcripts (VH-families, IgM, and IgD) in head kidney and spleen of the Atlantic cod (Gadus morhua L.)", DEV COMP IM, 25(4), 2001, pp. 291-302

Abstract

Expression of the immunoglobulin (Ig) heavy chain transcripts in spleen and head kidney of the Atlantic cod (Gadus morhua L.) was investigated using in situ hybridization (ISH) and northern blotting. Specific detection of plasma cells was done with a probe for secretory IgM transcripts (mu4). The plasma cells were often clustered close to blood vessels. Cells expressing surface IgM and IgD transcripts were detected using ISH with tyramide signalamplification (TSA). The positive cells were more abundant than plasma cells, had a lymphocyte-like morphology, and were evenly distributed throughout the tissues. This suggests that cod IgD mainly is expressed as a B-cell receptor akin to IgD in mammals. The VH-III family dominated the repertoire within the plasma cells, in agreement with data from cDNA cloning. Immunization with hapten-carrier antigen did not induce a systemic antibody response, and neither was any change in the clustering or distribution pattern of plasma cells within the tissues seen. A few clusters of plasma cells expressed only the rare VH-I and VH-II families, suggesting an ongoing clonal expansion and differentiation in these regions independently of immunization. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:03:06