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Titolo:
Novel methods for biocatalyst screening
Autore:
Wahler, D; Reymond, JL;
Indirizzi:
Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland Univ Bern BernSwitzerland CH-3012 & Biochem, CH-3012 Bern, Switzerland
Titolo Testata:
CURRENT OPINION IN CHEMICAL BIOLOGY
fascicolo: 2, volume: 5, anno: 2001,
pagine: 152 - 158
SICI:
1367-5931(200104)5:2<152:NMFBS>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATALYTIC ANTIBODY DETECTION; RESONANCE ENERGY-TRANSFER; IMMUNOASSAY CAT-EIA; DIRECTED EVOLUTION; FLUOROGENIC ASSAY; HIGH-THROUGHPUT; ENZYMATIC-ACTIVITY; HELPFUL TECHNIQUE; ENZYMES; LIBRARIES;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Wahler, D Univ Bern, Dept Chem & Biochem, Freie Str 3, CH-3012 Bern, Switzerland Univ Bern Freie Str 3 Bern Switzerland CH-3012 ern, Switzerland
Citazione:
D. Wahler e J.L. Reymond, "Novel methods for biocatalyst screening", CURR OP C B, 5(2), 2001, pp. 152-158

Abstract

There have been a number of recent advances in catalysis assays applicablefor screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, massspectrometry, capillary electrophoresis and IR-thermography, reagent-basedassays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 22:43:48