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Titolo:
Glycosidase activity in the post-ecdysial cuticle of the blue crab, Callinectes sapidus
Autore:
Roer, RD; Halbrook, KE; Shafer, TH;
Indirizzi:
Univ N Carolina, Dept Biol Sci, Wilmington, NC 28403 USA Univ N Carolina Wilmington NC USA 28403 iol Sci, Wilmington, NC 28403 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 4, volume: 128, anno: 2001,
pagine: 683 - 690
SICI:
1096-4959(200104)128:4<683:GAITPC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-N-ACETYLGLUCOSAMINIDASE; CRUSTACEAN CUTICLE; PURIFICATION; ACETYLHEXOSAMINIDASE; HEPATOPANCREAS; PROTEIN; SHRIMP; CYCLE;
Keywords:
glycosidase; N-acetylhexosaminidase; Callinectes; ecdysis; exoskeleton; glycoproteins; molting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Roer, RD Univ N Carolina, Dept Biol Sci, 601 S Coll Rd, Wilmington, NC 28403 USA Univ N Carolina 601 S Coll Rd Wilmington NC USA 28403 C 28403 USA
Citazione:
R.D. Roer et al., "Glycosidase activity in the post-ecdysial cuticle of the blue crab, Callinectes sapidus", COMP BIOC B, 128(4), 2001, pp. 683-690

Abstract

We have previously demonstrated a marked change in sugar moieties of glycoproteins of the cuticle of the blue crab, Callinectes sapidus, between 0.5 and 3 h post-ecdysis. The present study has identified a glycosidase that appears in the cuticle during the early post-ecdysial hours. The enzyme has affinities for p-nitrophenyl derivatives of both N-acetylglucosamine and N-acetylgalactosamine. Both activities are competitively inhibited by chitobiose, suggesting that the enzyme could be a N-acetylhexosaminidase (EC 3.2.1.52). Atypical of N-acetylhexosaminidases described to date, this enzyme has a pH optimum of 7.0. The enzyme activity is high during the post-ecdysialperiod coincident with the changes in glycoprotein profiles observed in vivo. Partial purification of the enzyme has been accomplished by Sephacryl size-exclusion chromatography followed by concanavalin A affinity chromatography. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:06:37