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Titolo:
Cellular uptake of taurine by lactating porcine mammary tissue
Autore:
Bryson, JM; Jackson, SC; Wang, H; Hurley, WL;
Indirizzi:
Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 is, Dept Anim Sci, Urbana, IL 61801 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 4, volume: 128, anno: 2001,
pagine: 667 - 673
SICI:
1096-4959(200104)128:4<667:CUOTBL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; HIGH-AFFINITY; CHLORIDE; PROTEIN; MILK; CLONING; CELLS; SOWS;
Keywords:
taurine; amino acid; transport; sow; lactation; mammary gland; milk; neonate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Hurley, WL 1207 W Gregory Dr, Urbana, IL 61801 USA 1207 W Gregory Dr Urbana IL USA 61801 Dr, Urbana, IL 61801 USA
Citazione:
J.M. Bryson et al., "Cellular uptake of taurine by lactating porcine mammary tissue", COMP BIOC B, 128(4), 2001, pp. 667-673

Abstract

Milk taurine plays a critical role in neonatal development. Taurine uptakein lactating sow mammary tissue has not been characterized previously. Thekinetic properties, ion dependence and substrate specificity of taurine uptake were characterized in mammary tissue collected from lactating sows at slaughter. Tissue explants were incubated in an isosmotic physiologic buffer with [H-3]taurine tracer to measure taurine uptake. Taurine uptake was dependent upon the presence of extracellular sodium and chloride ions, which is consistent with the co-transport of sodium and chloride with taurine. Uptake was not dependent upon ion exchange mechanisms or upon furosemide-sensitive ion co-transport. Taurine uptake was saturable and exhibited an apparent K-m of 20 muM and a V-max of 386 mu mol/kg cell water/30 min. Substratespecificity studies indicated a strong interaction of beta -amino acids with the taurine transport system. Taurine transport in lactating sow mammarytissue is therefore a high affinity, sodium-dependent mechanism specific for beta -amino acids, and is analogous to sodium-dependent taurine uptake in other tissues. The high affinity and high specificity of the taurine uptake system allows for concentration of taurine within the mammary cell and is ultimately responsible for provision of taurine required for neonatal development. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:30:28