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Titolo:
Specific combinations of G-protein subunits discriminate hormonal signalling in rat pituitary (GH(3)) cells in culture
Autore:
Johansen, PW; Lund, HW; Gordeladze, JO;
Indirizzi:
Univ Oslo, Inst Med Biochem, Oslo, Norway Univ Oslo Oslo NorwayUniv Oslo, Inst Med Biochem, Oslo, Norway Aker Hosp, Hormone Lab, Oslo, Norway Aker Hosp Oslo NorwayAker Hosp, Hormone Lab, Oslo, Norway
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 4, volume: 13, anno: 2001,
pagine: 251 - 256
SICI:
0898-6568(200104)13:4<251:SCOGSD>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-GAMMA-SUBUNITS; GTP-BINDING PROTEINS; ADENYLATE-CYCLASE; DOPAMINE-RECEPTORS; ALPHA-SUBUNIT; TUMOR CELLS; INHIBITION; TRANSDUCTION; THYROLIBERIN; STIMULATION;
Keywords:
dopamine; somatostatin; thyroliberin; vasoactive intestinal peptide; G protein alpha beta gamma heterotrimers; adenylate cyclase; phospholipase C; GH cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Johansen, PW Univ Oslo, Natl Hosp, Dept Clin Pharmacol, NO-0027 Oslo, Norway Univ Oslo Oslo Norway NO-0027 armacol, NO-0027 Oslo, Norway
Citazione:
P.W. Johansen et al., "Specific combinations of G-protein subunits discriminate hormonal signalling in rat pituitary (GH(3)) cells in culture", CELL SIGNAL, 13(4), 2001, pp. 251-256

Abstract

It was previously shown that hormone receptor coupling to voltage-dependent calcium channels in prolactin and growth hormone producing GH(3) cells was heavily dependent on the specific heterotrimeric combinations of alpha, beta, and gamma subunits of the guanosine triphosphate (GTP)-binding proteinfamily. Consequently, we assessed whether this was also the case for hormonal modulation of the adenylate cyclase (AC) and phospholipase C (PL-C) effector enzymes in GH(3) cells in culture. By employing polyclonal antibodiesdirected towards C-terminal decapeptides of various ol subunits in membrane assays, as well as antisense oligonucleotides towards certain beta and gamma subunit genes in whole-cell incubations, it was possible to unravel a tentative profile of heterotrimers preferred by some of the seven-transmembrane-stretch receptors in their modulation of AC and PL-C activities. Vasoactive intestinal peptide (VIP) and thyroliberin (TRH) activate membrane-bound AC through alpha (s)beta (2)gamma (2), while somatostatin (SRM) and dopamine (DA) inhibited the AC through alpha (i2)beta (1)gamma (3). TRH activated membrane-bound PL-C through alpha (q/11)beta (4)gamma (2), while DA inhibition of the PL-C was accomplished via alpha (0)beta (3)gamma (1). Hence, it seems that not only the specificity of alpha subunits determines the coupling between G protein-associated receptors in GH cells, the receptor binding to G proteins also requires certain combinations of beta and gamma subunits. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:37:23