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Titolo:
Modulation of CREB and NF-kappa B signal transduction by cannabinol in activated thymocytes
Autore:
Herring, AC; Kaplan, BLF; Kaminski, NE;
Indirizzi:
Michigan State Univ, Dept Pharmacol & Toxicol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 xicol, E Lansing, MI 48824 USA Michigan State Univ, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 l Ctr, E Lansing, MI 48824 USA
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 4, volume: 13, anno: 2001,
pagine: 241 - 250
SICI:
0898-6568(200104)13:4<241:MOCANB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELEMENT-BINDING PROTEIN; CYCLIC-AMP; ADENYLATE-CYCLASE; RESPONSE ELEMENT; T-LYMPHOCYTES; KINASE-C; GENE-EXPRESSION; IKK-ALPHA; INHIBITION; PHOSPHORYLATION;
Keywords:
CREB; NF-kappa B; cannabinol; thymocytes; cAMP; interleukin-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kaminski, NE Michigan State Univ, Dept Pharmacol & Toxicol, 315 Food Safety & Toxicol Bldg, E Lansing, MI 48824 USA Michigan State Univ 315 Food Safety & Toxicol Bldg E Lansing MI USA 48824
Citazione:
A.C. Herring et al., "Modulation of CREB and NF-kappa B signal transduction by cannabinol in activated thymocytes", CELL SIGNAL, 13(4), 2001, pp. 241-250

Abstract

Cannabinoid compounds inhibit the cAMP signalling cascade in leukocytes. One of these compounds, cannabinol (CBN has been shown to inhibit interleukin-2 (IL-2) expression and the activation of cAMP response element binding protein (CREB) and nuclear factor for immunoglobulin re chain in B cells (NF-kappaB) following phorbol-12-myristate-13 acetate (PMA) plus ionomycin Oo)treatment of thymocytes. Therefore, the objective of the present studies was to determine the role of cAMP and protein kinase A (PKA) in the CBN-mediated inhibition of IL-2, CREB, and NF-kappaB in PMA/Io-activated thymocytes. The inhibition of CREB/ATF-1 phosphorylation, or cAMP response element (CRE) or KB DNA binding activity produced by CBN in PMA/Io-activated thymocytes, could not be reversed by DBcAMP costimulation. Furthermore, DBcAMP failed to reverse the concentration-dependent inhibition of IL-2 protein secretion by CBN. Pretreatment of thymocytes with H89 produced a modest inhibition of PMA/Io-induced CREB/ATF-1 phosphorylation and CRE DNA binding activitybut H89 had no effect on protein binding to a kappaB motif. Additionally, H89 modestly inhibited PMA/Io-induced IL-2 secretion. In light of the modest involvement of the cAMP pathway in CBN-mediated inhibition of CREB and IL-2 in PMA/Io-activated thymocytes, PD098059 (PD), the MEK inhibitor, was utilized to determine the role of ERK MAP kinases in thymocytes. ERKs play a critical role in IL-2 production but not for CREB phsophorylation. Collectively these findings suggest that CBN may modulate several signalling pathways in activated T cells. (C) 2001 Elsevier Science Inc. All rights reserved.

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Documento generato il 18/09/20 alle ore 17:03:54