Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
cGMP serves as an extracellular regulator of a Ca2+-dependent K+ channel in immortalized human proximal tubule cells
Autore:
Hirsch, JR; Weber, G; Kleta, I; Schlatter, E;
Indirizzi:
Univ Munster, Med Poliklin, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 Poliklin, D-48149 Munster, Germany
Titolo Testata:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 2, volume: 11, anno: 2001,
pagine: 77 - 82
SICI:
1015-8987(2001)11:2<77:CSAAER>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
KIDNEY EPITHELIAL-CELLS; ATRIAL NATRIURETIC FACTOR; ACID-TRANSPORT-SYSTEMS; CYCLIC-NUCLEOTIDES; RAT-KIDNEY; INHIBITION; DIURESIS; IHKE-1;
Keywords:
extracellular regulation; cyclic nucleotides; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Hirsch, JR Univ Munster, Med Poliklin, Domagkstr 3A, D-48149 Munster, Germany Univ Munster Domagkstr 3A Munster Germany D-48149 ter, Germany
Citazione:
J.R. Hirsch et al., "cGMP serves as an extracellular regulator of a Ca2+-dependent K+ channel in immortalized human proximal tubule cells", CELL PHYS B, 11(2), 2001, pp. 77-82

Abstract

Recently we showed that a K+ channel in immortalized human kidney epithelial (IHKE-1) cells derived from the proximal tubule is regulated by natriuretic peptides in cell-attached patches and directly regulated by cGMP in excised inside-out oriented membrane patches [1], The patch clamp technique was used to investigate the regulatory effect of extracellular, nonmembrane permeable cGMP on membrane voltage and the regulation of this K+ channel in outside-out oriented membrane patches. In 7 out of 7 experiments the membrane voltage of IHKE-1 cells depolarized by 3.9 +/- 0.1 mV when the non-membrane permeable cGMP was added to the bath solution. In outside-out oriented membrane patches cGMP inhibited P already at 1 muM (-12 +/- 4%, n=7), at 0.1 mM inhibition of P reached -39 +/- 6% (n=14). cAMP (0.1 mM) only had Da weak inhibitory effect (n=7). GTP and ATP (n=7 each) had no significant effect on P-o from the outside. When cGMP was added to the pipette solution in experiments with outside-out oriented membranes cGMP still inhibited this K channel from the outside by 36 +/- 6% (n=6). In 4 paired experiments 8-Br-cGMP (0.1 mM) showed a significantly higher inhibitory effect on P-o compared to cGMP (0.1 mM). cGMP inhibits a K+ channel in human proximal tubule cells from the outside and may serve as an autocrine and paracrine regulatory factor in the kidney. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:54:51