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Titolo:
Molecular regulation of tumor cell vasculogenic mimicry by tyrosine phosphorylation: Role of epithelial cell kinase (Eck/EphA2)
Autore:
Hess, AR; Seftor, EA; Gardner, LMG; Carles-Kinch, K; Schneider, GB; Seftor, REB; Kinch, MS; Hendrix, MJC;
Indirizzi:
Univ Iowa, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 nat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa, Holden Comprehens Canc Ctr, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 rehens Canc Ctr, Iowa City, IA 52242 USA Univ Iowa, Coll Dent, Dow Inst Dent Res, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 w Inst Dent Res, Iowa City, IA 52242 USA Univ Iowa, Coll Dent, Dept Prosthodont, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 ept Prosthodont, Iowa City, IA 52242 USA Purdue Univ, Dept Basic Med Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 c Med Sci, W Lafayette, IN 47907 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 8, volume: 61, anno: 2001,
pagine: 3250 - 3255
SICI:
0008-5472(20010415)61:8<3250:MROTCV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOMA PROGRESSION; HUMAN CYTOTROPHOBLASTS; ENDOVASCULAR INVASION; GENE-EXPRESSION; UP-REGULATION; CDNA CLONING; PROTEIN; ADHESION; ECK; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hendrix, MJC Univ Iowa, Dept Anat & Cell Biol, 51 Newton Rd,1-100 Bowen Sci Bldg, Iowa City, IA 52242 USA Univ Iowa 51 Newton Rd,1-100 Bowen Sci BldgIowa City IA USA 52242
Citazione:
A.R. Hess et al., "Molecular regulation of tumor cell vasculogenic mimicry by tyrosine phosphorylation: Role of epithelial cell kinase (Eck/EphA2)", CANCER RES, 61(8), 2001, pp. 3250-3255

Abstract

During embryogenesis, blood vessels are formed initially by the process ofvasculogenesis, the in situ differentiation of mesenchymal cells into endothelial cells, which form a primitive, patterned vasculogenic network This is followed by angiogenesis, the sprouting of nea vessels from preexisting vasculature, to yield a more refined microcirculation. However, we and our collaborators have recently described a process termed "vasculogenic mimicry," which consists of the formation of patterned, tubular networks by aggressive melanoma tumor cells (in three-dimensional cultures in vitro), that mimics endothelial-formed vasculogenic networks and correlates dth poor clinical prognosis in patients. Previous microarray analysis from our laboratory comparing the highly aggressive versus the poorly aggressive melanoma cells revealed a significant increased expression of tyrosine kinases associated with the aggressive melanoma phenotype. Because of the important role ofprotein tyrosine kinases in phosphorylating various signal transduction proteins that are critical for many cellular processes (e.g., cell adhesion, migration, and invasion), we examined whether protein tyrosine kinases are involved in melanoma vasculogenic mimicry. Immunofluorescence analysis of aggressive melanoma cells forming tubular networks in vitro showed that tyrosine phosphorylation activity colocalized specifically within areas of tubular network formation, A phosphotyrosine profile of the aggressive melanomacells capable of forming tubular networks indicated differences in tyrosine phosphorylated proteins compared with the poorly aggressive melanoma cells (incapable of forming tubular networks),,Most notably, we identified epithelial cell kinase (EphA2) as being one receptor tyrosine kinase expressed and phosphorylated exclusively in the aggressive metastatic melanoma cells,Furthermore, general inhibitors of protein tyrosine kinases hindered tube formation, and transient knockout of EphA2 abrogated the ability of tumor cells to form tubular structures. These results suggest that protein tyrosine kinases, particularly EphA2, are involved in the formation of tubular networks by aggressive melanoma tumor cells in vitro, which may represent a novel therapeutic target for further clinical investigation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 13:48:52