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Titolo:
Identification and quantitation of steroid hormones in marine gastropods by GC/MS
Autore:
Lu, M; Horiguchi, T; Shiraishi, H; Shibata, Y; Abo, M; Okubo, A; Yamazaki, S;
Indirizzi:
Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 iol Chem, Bunkyo Ku, Tokyo 1138657, Japan Natl Inst Environm Studies, Environm Chem Div, Tsukuba, Ibaraki 3050053, Japan Natl Inst Environm Studies Tsukuba Ibaraki Japan 3050053 i 3050053, Japan
Titolo Testata:
BUNSEKI KAGAKU
fascicolo: 4, volume: 50, anno: 2001,
pagine: 247 - 255
SICI:
0525-1931(200104)50:4<247:IAQOSH>2.0.ZU;2-P
Fonte:
ISI
Lingua:
JPN
Soggetto:
SEMINAL-VESICLE FLUID; CYNOMOLGUS MONKEY; MACACA-FASCICULARIS; ORGANOTIN COMPOUNDS; CLARIAS-GARIEPINUS; THAIS-CLAVIGERA; AFRICAN CATFISH; IN-VITRO; TESTOSTERONE; IMPOSEX;
Keywords:
testosterone; estradiol; GC/MS; Thais clavigera; Babylonia japonica;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, M Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 oi, Tokyo 1138657, Japan
Citazione:
M. Lu et al., "Identification and quantitation of steroid hormones in marine gastropods by GC/MS", BUNSEKI KAG, 50(4), 2001, pp. 247-255

Abstract

In order to clarify the effect of the contamination of organotin compoundsin a marine environment oil the imposex of gastropods, the speciation and levels of steroid hormones of the organisms were investigated by means of the GC/MS method. Testosterone and 17 beta -estradiol were first identified and quantitated in the testes and ovaries of Thais clavigera, respectively. In addition, androsterone, estrone and ethynlestradiol were also found in the respective tissues of Babylonia japonica. The extraction and refining procedures were constructed, and the passage of C-8-SPE was quite effective for clean-up of the steroid fractions. The recovery of testosterone and 17 beta -estradiol through the whole procedure were examined by adding a surrogate compound, 17 beta -estradiol-d(3). Good recoveries of between 83% ana 96% were obtained. Based on these, the concentrations of testosterone in the testes and 17 beta -estradiol in the ovaries of both marine gastropods were well determined. The testes of Babylonia japonica were stained both histologically and immunohistochemically to observe the presence of inter- stitial cells, testosterone producing cells known in mammals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:47:01