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Titolo:
Sexing chick embryos: a rapid and simple protocol
Autore:
Clinton, M; Haines, L; Belloir, B; McBride, D;
Indirizzi:
Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland Roslin Inst Roslin Midlothian Scotland EH25 9PS 9PS, Midlothian, Scotland
Titolo Testata:
BRITISH POULTRY SCIENCE
fascicolo: 1, volume: 42, anno: 2001,
pagine: 134 - 138
SICI:
0007-1668(200103)42:1<134:SCEARA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SRY-RELATED GENE; GALLUS-G-DOMESTICUS; CAMPOMELIC DYSPLASIA; SEQUENCES; BIRDS; CONSERVATION; EXPRESSION; MUTATIONS; REVERSAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Clinton, M Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland Roslin InstRoslin Midlothian Scotland EH25 9PS hian, Scotland
Citazione:
M. Clinton et al., "Sexing chick embryos: a rapid and simple protocol", BR POULT SC, 42(1), 2001, pp. 134-138

Abstract

1. Analysis of gene expression in the developing chick gonads requires thecollection of male and female tissues from embryos between 3.5 d and 8.5 dof development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development.2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming.3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA.4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:46:41