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Titolo:
Calcium-induced changes in chondroitin sulfate chains of urinary trypsin inhibitor
Autore:
Masui, M; Suzuki, M; Fujise, Y; Kanayama, N;
Indirizzi:
Hamamatsu Univ, Sch Med, Dept Obstet & Gynecol, Hamamatsu, Shizuoka 4313192, Japan Hamamatsu Univ Hamamatsu Shizuoka Japan 4313192 , Shizuoka 4313192, Japan Hamamatsu Univ, Sch Med, Equipment Ctr, Hamamatsu, Shizuoka 4313192, JapanHamamatsu Univ Hamamatsu Shizuoka Japan 4313192 , Shizuoka 4313192, Japan Hamamatsu Univ, Sch Med, Dept Chem, Hamamatsu, Shizuoka 4313192, Japan Hamamatsu Univ Hamamatsu Shizuoka Japan 4313192 , Shizuoka 4313192, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 2, volume: 1546, anno: 2001,
pagine: 261 - 267
SICI:
0167-4838(20010407)1546:2<261:CCICSC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTER-ALPHA-INHIBITOR; HUMAN-PLASMA; PROTEIN; SERUM; CELLS; EXPRESSION; ELASTASE; RELEASE; FAMILY; GENE;
Keywords:
urinary trypsin inhibitor; chondroitin sulfate chain; calcium ion; proton nuclear magnetic resonance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Masui, M Hamamatsu Univ, Sch Med, Dept Obstet & Gynecol, 3600 Handa Cho, Hamamatsu,Shizuoka 4313192, Japan Hamamatsu Univ 3600 Handa Cho Hamamatsu Shizuoka Japan 4313192 an
Citazione:
M. Masui et al., "Calcium-induced changes in chondroitin sulfate chains of urinary trypsin inhibitor", BBA-PROT ST, 1546(2), 2001, pp. 261-267

Abstract

Urinary trypsin inhibitor (UTI) has several roles other than protease inhibition. It is suggested that UTI inhibits calcium influx in cultured cells and that the chondroitin sulfate chain of UTI may play an important role. In order to clarify the mechanistic features of this phenomenon, the chondroitin sulfate chain of UTI was analyzed by H-1-NMR. The samples were highly purified UTI dissolved in D2O in the presence or absence of Ca2+, Mg2+ and Na+. 1D-spectra were obtained and T-1 values of detected signals were estimated from the inversion-recovery method. The addition of Ca2+ to UTI causeda chemical shift to downfield, line broadening and a reduction of T-1 values at several signals from chondroitin sulfate moiety (especially at axial H-2 of GalNAc), whereas Mg2+ and Naf had no significant effect. Some of thesignals in the linkage region of chondroitin sulfate chain showed marked line broadening by Ca2+. The reduction of T1 values implies formation of a complex. It is suggested that Ca2+ generates the sulfate salt and interacts with other polar groups in the chondroitin sulfate chain, thereby causing bridging between UTI molecules. Several properties of UTI may be related to this interaction of Ca2+ with chondroitin sulfate chains. (C) 2001 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:22:53