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Titolo:
Immunochemical and catalytic characterization of hepatic microsomal cytochrome P450 in the sperm whale (Physeter macrocephalus)
Autore:
Boon, JP; Lewis, WE; Goksoyr, A;
Indirizzi:
Netherlands Inst Sea Res, NL-1790 AB Den Burg, Netherlands Netherlands Inst Sea Res Den Burg Netherlands NL-1790 AB rg, Netherlands Univ Bergen, HIB, Dept Mol Biol, N-5020 Bergen, Norway Univ Bergen Bergen Norway N-5020 B, Dept Mol Biol, N-5020 Bergen, Norway
Titolo Testata:
AQUATIC TOXICOLOGY
fascicolo: 3-4, volume: 52, anno: 2001,
pagine: 297 - 309
SICI:
0166-445X(200105)52:3-4<297:IACCOH>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELPHINAPTERUS-LEUCAS; LIVER-MICROSOMES; MARINE MAMMALS; METABOLISM; ORGANOCHLORINES; TOXAPHENE(R); CETACEANS; COAST;
Keywords:
Physeter macrocephalus; sperm whale; cytochrome P450; biotransformation; persistent organic pollutants; PHAHs; PCBs; chlorobornanes; toxaphene (R);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Boon, JP Netherlands Inst Sea Res, POB 59, NL-1790 AB Den Burg, Netherlands Netherlands Inst Sea Res POB 59 Den Burg Netherlands NL-1790 AB
Citazione:
J.P. Boon et al., "Immunochemical and catalytic characterization of hepatic microsomal cytochrome P450 in the sperm whale (Physeter macrocephalus)", AQUAT TOX, 52(3-4), 2001, pp. 297-309

Abstract

Liver samples from three live-stranded adult male sperm whales, that couldbe sampled and frozen in liquid nitrogen within 18 h post mortem, providedan opportunity to learn more about the basic properties of their cytochrome P450 (CYP) system. All samples were catalytically active and showed sharpbands of the different CYP enzymes after Western blotting, indicating thatdegradation of the proteins was negligible. All three sperm whales showed a similar immunochemical CYP pattern: bands of CYP1A1/2, CYP3A and CYP4A were present, but CYP3B1/2 was not detected. Significant biotransformation ofthe polychlorinated aromatic hydrocarbons 4, 4'-dichlorobiphenyl (CB-15), 2,7-dichlorodibenzodioxin and 1,2,3,4,8-pentadibenzofuran was measured in an in vitro biotransformation assay. In contrast, 3,3',4,4'-tetrachlorobiphenyl (CB-77) and two chlorobornanes (CHB-32 and CHB-62) occurring in the insectide toxaphene(R), were not metabolised. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 06:41:29