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Titolo:
Effect of bafilomycin A1, a specific inhibitor of vacuolar (V-type) protonATPases, on the capacitation of rabbit spermatozoa
Autore:
Garcia-Macedo, R; Rosales, AM; Hernandez-Perez, O; Chavarria, ME; Reyes, A; Rosado, A;
Indirizzi:
Inst Mexianco Seguro Social, CMNS XX1, Hosp Especialidades, Unidad Invest Med Bioquim, Mexico City 06725, DF, Mexico Inst Mexianco Seguro Social Mexico City DF Mexico 06725 06725, DF, Mexico Hosp Gineco Obstet Luis Castelazo Ayala, Unidad Invest Med Med Reprod, Inst Mexicano Seguro Social, Mexico City, DF, Mexico Hosp Gineco Obstet Luis Castelazo Ayala Mexico City DF Mexico DF, Mexico
Titolo Testata:
ANDROLOGIA
fascicolo: 2, volume: 33, anno: 2001,
pagine: 113 - 121
SICI:
0303-4569(200103)33:2<113:EOBAAS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM ACROSOME REACTION; INTRACELLULAR PH; BOVINE SPERM; FERTILIZING ABILITY; MAMMALIAN SPERM; NA+/H+ EXCHANGE; MOUSE SPERM; MOTILITY; REQUIREMENT; MICROSCOPY;
Keywords:
acrosome reaction; bafilomycin; capacitation; rabbit spermatozoa; V-type H+-ATPase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Macedo, R Inst Mexianco Seguro Social, CMNS XX1, Hosp Especialidades, Unidad Invest Med Bioquim, Av Cuauhtemoc 330, Mexico City 06725, DF, Mexico Inst Mexianco Seguro Social Av Cuauhtemoc 330 Mexico City DF Mexico 06725
Citazione:
R. Garcia-Macedo et al., "Effect of bafilomycin A1, a specific inhibitor of vacuolar (V-type) protonATPases, on the capacitation of rabbit spermatozoa", ANDROLOGIA, 33(2), 2001, pp. 113-121

Abstract

Mammalian spermatozoa maintain precisely regulated ionic gradients that must be modified during capacitation and the acrosome reaction. In other celltypes, ionic gradients are mainly regulated by the presence in plasma membranes of three metabolically different types of ATPases. The modifications induced during in vitro capacitation of rabbit spermatozoa by the specific inhibition of V-type H+-ATPases with bafilomycin A were studied. We used chlortetracycline binding to rabbit spermatozoa to monitor capacitation, and the coomassie brilliant blue method to identify acrosome-reacted sperm cells. There was a significant difference between the percentage of epididymal (66 +/- 7%) and ejaculated (43 +/- 11%) spermatozoa capacitated in vitro, after a 6-h incubation period in the presence of Ca2+ without ATPase inhibitor. The presence of bafilomycin significantly reduced these numbers (25 +/-11 and 16 +/-8%, epididymal and ejaculated spermatozoa, respectively) and eliminated the difference. Ejaculated spermatozoa capacitated in the absence of bafilomycin showed a linear increase in the percentage of acrosome reactions induced by the addition of A23187 (12 +/-5, 23 +/-6 and 31 +/-5 after 15, 30 and 45 min). The presence of 0.2 mu mol l(-1) bafilomycin during the capacitation incubation induced a significant decrease in the acrosome reaction percentages (4 +/-2, 8 +/-3 and 14 +/-4 after 15, 30 and 45 min). The addition of bafilomycin after the capacitating period had no effect uponthe induction of the acrosome reaction by A23187. These results indicate that vacuolar ATPases play an important role during rabbit sperm capacitation. However, once the spermatozoa have been capacitated, V-type ATPases do not have a significant participation during the acrosome reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:32:23