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Titolo:
A CE-MALDI interface based on the use of prestructured sample supports
Autore:
Johnson, T; Bergquist, J; Ekman, R; Nordhoff, E; Schurenberg, M; Kloppel, KD; Muller, M; Lehrach, H; Gobom, J;
Indirizzi:
Univ Gothenburg, SU Molndal, Inst Clin Neurosci, SE-43180 Molndal, Sweden Univ Gothenburg Molndal Sweden SE-43180 urosci, SE-43180 Molndal, Sweden Univ Uppsala, SE-75121 Uppsala, Sweden Univ Uppsala Uppsala Sweden SE-75121 v Uppsala, SE-75121 Uppsala, Sweden Max Planck Inst Mol Genet, D-14195 Berlin, Germany Max Planck Inst Mol Genet Berlin Germany D-14195 D-14195 Berlin, Germany Bruker Dalton, D-28359 Bremen, Germany Bruker Dalton Bremen Germany D-28359 ker Dalton, D-28359 Bremen, Germany
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 8, volume: 73, anno: 2001,
pagine: 1670 - 1675
SICI:
0003-2700(20010415)73:8<1670:ACIBOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED-LASER-DESORPTION/IONIZATION; CAPILLARY-ZONE-ELECTROPHORESIS; IONIZATION MASS-SPECTROMETRY; MATRIX; PROTEINS; IDENTIFICATION; PEPTIDES; TECHNOLOGY; DEPOSITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, T Univ Gothenburg, SU Molndal, Inst Clin Neurosci, SE-43180 Molndal, Sweden Univ Gothenburg Molndal Sweden SE-43180 43180 Molndal, Sweden
Citazione:
T. Johnson et al., "A CE-MALDI interface based on the use of prestructured sample supports", ANALYT CHEM, 73(8), 2001, pp. 1670-1675

Abstract

We have developed an off-line coupling of capillary electrophoresis (CE) to matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on CE fraction collection onto prestructured MALDI sample supports. Analyte carryover and detection sensitivity were investigated using a standard peptide mixture. Low femtomole amounts were detected, and no noticeable carryover was discovered. The performance of the methodwas evaluated with a mixture of tryptic digests of proteins from a human fetal brain cDNA expression library. The total number of identified peptideswas increased from 47 to 211 when the CE-MALDI interface was used comparedto direct MALDI-MS analysis. Sequence coverage with CE-MALDI was in the 25-60% range for the different proteins, corresponding to an increase of 1.3-4.9 times relative to that obtained with MALDI-MS of the crude mixture. Fractionation of sample components also facilitated protein identification by MAG DI postsource decay analysis. Our initial results suggest this CE-MALDIinterface can be used for the analysis of complex peptide mixtures isolated from biological tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:08:35