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Titolo:
MEK inhibits secretin release and pancreatic secretion: roles of secretin-releasing peptide and somatostatin
Autore:
Li, JP; Lee, KY; Chang, TM; Chey, WY;
Indirizzi:
Univ Rochester, Med Ctr, Konar Ctr Digest & Liver Dis, Rochester, NY 14642USA Univ Rochester Rochester NY USA 14642 & Liver Dis, Rochester, NY 14642USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: G890 - G896
SICI:
0193-1857(200105)280:5<G890:MISRAP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE INTESTINAL POLYPEPTIDE; GASTRIC-ACID SECRETION; SUBSTANCE-P; GASTROINTESTINAL-TRACT; EXOCRINE SECRETION; ANESTHETIZED RATS; ENKEPHALIN; MECHANISM; NEURONS; INSULIN;
Keywords:
naloxone; anti-somatostatin serum; rats; fluid; bicarbonate secretion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Chey, WY Rochester Inst Digest Dis & Sci, 222 Alexander St,Suite 3100, Rochester, NY 14607 USA Rochester Inst Digest Dis & Sci 222 Alexander St,Suite3100 Rochester NY USA 14607
Citazione:
J.P. Li et al., "MEK inhibits secretin release and pancreatic secretion: roles of secretin-releasing peptide and somatostatin", AM J P-GAST, 280(5), 2001, pp. G890-G896

Abstract

We investigated the mechanism of action of methionine enkephalin (MEK) on HCl-stimulated secretin release and pancreatic exocrine secretion. Anesthetized rats with pancreatobiliary cannulas and isolated upper small intestinal loops were perfused intraduodenally with 0.01 N HCl while bile and pancreatic juice were diverted. The effect of intravenous MEK on acid-stimulated secretin release and pancreatic exocrine secretion was then studied with orwithout coinfusion of naloxone, an anti-somatostatin (SS) serum, or normalrabbit serum. Duodenal acid perfusate, which contains secretin-releasing peptide (SRP) activity, was collected from donor rats with or without pretreatment with MEK, MEK + naloxone, or MEK + anti-SS serum, concentrated by ultrafiltration, and neutralized. The concentrated acid perfusate (CAP), which contains SRP bioactivity, was infused intraduodenally into recipient rats. MEK increased plasma SS concentration and inhibited secretin release and pancreatic fluid and bicarbonate secretion dose-dependently. The inhibitionwas partially reversed by naloxone and anti-SS serum but not by normal rabbit serum. In recipient rats, CAP increased plasma secretin level and pancreatic secretion. CAP SRP bioactivity decreased when it was collected from MEK-treated donor rats; this was partially reversed by coinfusion with naloxone or anti-SS serum. These results suggest that in the rat, MEK inhibitionof acid-stimulated pancreatic secretion and secretin release involves suppression of SRP activity release. Thus the MEK inhibitory effect appears to be mediated in part by endogenous SS.

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Documento generato il 04/06/20 alle ore 01:11:17