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Titolo:
Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs
Autore:
Davis, TA; Fiorotto, ML; Beckett, PR; Burrin, DG; Reeds, PJ; Wray-Cahen, D; Nguyen, HV;
Indirizzi:
Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Nutr Res Ctr, Houston, TX 77030 USA Baylor Coll Med, Dept Pediat, Endocrinol & Metab Sect, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 & Metab Sect, Houston, TX 77030 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 5, volume: 280, anno: 2001,
pagine: E770 - E779
SICI:
0193-1849(200105)280:5<E770:DEOIOP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKELETAL-MUSCLE; WHOLE-BODY; AMINO-ACIDS; SUCKLING RATS; LEUCINE KINETICS; SYNTHESIS INVIVO; FOOD-INTAKE; LIVER; METABOLISM; HYPERINSULINEMIA;
Keywords:
neonate; insulin action; amino acids; protein synthesis; nutrition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Davis, TA Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston,TX 77030 USA Baylor Coll Med 1100 Bates St Houston TX USA 77030 ,TX 77030 USA
Citazione:
T.A. Davis et al., "Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs", AM J P-ENDO, 280(5), 2001, pp. E770-E779

Abstract

We recently demonstrated in neonatal pigs that, with amino acids and glucose maintained at fasting levels, the stimulation of protein synthesis in longissimus dorsi muscle with feeding can be reproduced by a physiological rise in insulin alone. In the current report, we determine whether the response of protein synthesis to insulin in the neonatal pig is 1) present In muscles of different fiber types, 2) proportional in myofibrillar and sarcoplasmic proteins, 3) associated with increased translational efficiency and ribosome number, and 4) present in other peripheral tissues and in viscera. Hyperinsulinemic-euglycemic-amino acid clamps were performed in 7- and 26-day-old pigs infused with 0, 30, 100, or 1,000 ng.kg(-0.66).min(-1) of insulin to reproduce insulin levels present in fasted, fed, refed, and supraphysiological conditions, respectively. Tissue protein synthesis was measured using a flooding dose of L-[4-H-3]phenylalanine. Insulin increased protein synthesis in gastrocnemius muscle and, to a lesser degree, masseter muscle. The degree of stimulation of protein synthesis by insulin was similar in myofibrillar and sarcoplasmic proteins. Insulin increased translational efficiency but had no effect on ribosome number in muscle. AU of these insulin-induced changes in muscle protein synthesis decreased with age. Insulin also stimulated protein synthesis in cardiac muscle and skin but not in liver, intestine, spleen, pancreas, or kidney. The results support the hypothesis that insulin mediates the feeding-induced stimulation of myofibrillar and sarcoplasmic protein synthesis in muscles of different fiber types in the neonate by increasing the efficiency of translation. However, insulin does notappear to be involved in the feeding-induced stimulation of protein synthesis in visceral tissues. Thus different mechanisms regulate the growth of peripheral and visceral tissues in the neonate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:42:28