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Titolo:
Influence of caveolin-1 on cellular cholesterol efflux mediated by high-density lipoproteins
Autore:
Frank, PG; Galbiati, F; Volonte, D; Razani, B; Cohen, DE; Marcel, YL; Lisanti, MP;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Dept Biochem, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NYUSA 10461 nx, NY 10461 USA Univ Ottawa, Inst Heart, Lipoprot & Atherosclerosis Grp, Ottawa, ON K1Y 4W7, Canada Univ Ottawa Ottawa ON Canada K1Y 4W7 osis Grp, Ottawa, ON K1Y 4W7, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: C1204 - C1214
SICI:
0363-6143(200105)280:5<C1204:IOCOCC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOLIPOPROTEIN-A-I; BINDING CASSETTE TRANSPORTER-1; PROTEIN-KINASE-C; ONCOGENICALLY TRANSFORMED-CELLS; PHOSPHOLIPID TRANSFER PROTEIN; PLASMA-MEMBRANE CAVEOLAE; CORONARY HEART-DISEASE; MESSENGER-RNA LEVELS; ROUS-SARCOMA VIRUS; TANGIER-DISEASE;
Keywords:
caveolin; oxidized low-density lipoprotein; sterol regulatory element-binding proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
82
Recensione:
Indirizzi per estratti:
Indirizzo: Lisanti, MP Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, 1300 Morris Pk Ave, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave Bronx NY USA 10461
Citazione:
P.G. Frank et al., "Influence of caveolin-1 on cellular cholesterol efflux mediated by high-density lipoproteins", AM J P-CELL, 280(5), 2001, pp. C1204-C1214

Abstract

Caveolin-1 is a principal structural component of caveolae membranes. These membrane microdomains participate in the regulation of signaling, transcytosis, and cholesterol homeostasis at the plasma membrane. In the present study, we determined the effect of caveolin-1 expression on cellular cholesterol efflux mediated by high-density lipoprotein (HDL). We evaluated this effect in parental NIH/3T3 cells as well as in two transformed NIH/3T3 cell lines in which caveolin-1 protein levels are dramatically downregulated. Compared with parental NIH/3T3 cells, these two transformed cell lines effluxed cholesterol more rapidly to HDL. In addition, NIH/3T3 cells harboring caveolin-1 antisense also effluxed cholesterol more rapidly to HDL. However, this effect was not due to changes in total cellular cholesterol content. We further showed that chronic HDL exposure reduced caveolin-1 protein expression in NIH/3T3 cells. HDL exposure also inhibited caveolin-1 promoter activity, suggesting a direct negative effect of HDL on caveolin-1 gene transcription. Moreover, we showed that HDL-induced downregulation of caveolin-1 prevents the uptake of oxidized low-density lipoprotein in human endothelial cells. These data suggest a novel proatherogenic role for caveolin-1, i.e., regarding the uptake and/or transcytosis of modified lipoproteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 12:39:54