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Titolo:
Nongenomic effect of testosterone on chloride secretion in cultured rat efferent duct epithelia
Autore:
Leung, GPH; Cheng-Chew, SB; Wong, PYD;
Indirizzi:
Chinese Univ Hong Kong, Fac Med, Dept Physiol, Shatin, Hong Kong, Peoples R China Chinese Univ Hong Kong Shatin Hong Kong Peoples R China Peoples R China
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: C1160 - C1167
SICI:
0363-6143(200105)280:5<C1160:NEOTOC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-H+ EXCHANGE; ANDROGEN RECEPTOR; CALCIUM-CONCENTRATION; FLUID REABSORPTION; PLASMA-MEMBRANES; ANION SECRETION; G-PROTEIN; T-CELLS; TRANSPORT; INHIBITION;
Keywords:
testosterone; chloride secretion; efferent duct;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, PYD Chinese Univ Hong Kong, Fac Med, Dept Physiol, Shatin, Hong Kong, Peoples R China Chinese Univ Hong Kong Shatin Hong Kong Peoples R China China
Citazione:
G.P.H. Leung et al., "Nongenomic effect of testosterone on chloride secretion in cultured rat efferent duct epithelia", AM J P-CELL, 280(5), 2001, pp. C1160-C1167

Abstract

Short-circuit current (Isc) technique was used to investigate the role of testosterone in the regulation of chloride secretion in cultured rat efferent duct epithelia. Among the steroids tested, only testosterone, and to a lesser extent, 5 alpha -dihydrotestosterone (5 alpha -DHT), reduced the basal and forskolin-induced Isc in cultured rat efferent duct epithelia when added to the apical bathing solution. Indomethacin, a 3 alpha -hydroxysteroiddehydrogenase, did not affect the inhibitory effect of 5 alpha -DHT. The effect of testosterone occurred within 10-20 s upon application and was dosedependent with apparent IC50 value of 1 muM. The effect was abolished by removal of Cl- but not HCO3- from the normal Krebs-Henseleit solution, suggesting that testosterone mainly inhibited Cl- secretion. The efferent duct was found to be most sensitive to testosterone, while the caput and the cauda epididymidis were only mildly sensitive. Cyproterone acetate, a steroidalantiandrogen, or flutamide, a nonsteroidal antiandrogen, did not block theeffect of testosterone on the forskolin-induced Isc, nor did protein synthesis inhibitors, cycloheximide, or actinomycin D. However, pertussis toxin,a G(i) protein inhibitor, attenuated the inhibition of forskolin-induced Isc by testosterone. Testosterone caused a dose-dependent inhibition of forskolin-induced rise in cAMP in efferent duct cells. It is suggested that therapid effect of testosterone was mediated through a membrane receptor thatis negatively coupled to adenylate cyclase via G(i) protein. The role of nongenomic action of testosterone in the regulation of electrolyte and fluidtransport in the efferent duct is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:42:06