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Titolo:
Increased contractility and altered Ca2+ transients of mouse heart myocytes conditionally expressing PKC beta
Autore:
Huang, L; Wolska, BM; Montgomery, DE; Burkart, EM; Buttrick, PM; Solaro, RJ;
Indirizzi:
Univ Illinois, Coll Med, Dept Physiol & Biophys M C 901, Chicago, IL 60612USA Univ Illinois Chicago IL USA 60612 Biophys M C 901, Chicago, IL 60612USA Univ Illinois, Coll Med, Dept Med, Sect Cardiol,Program Cardiovasc Sci, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Cardiovasc Sci, Chicago, IL 60612 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: C1114 - C1120
SICI:
0363-6143(200105)280:5<C1114:ICAACT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CARDIAC TROPONIN-I; SARCOPLASMIC-RETICULUM; DIFFERENTIAL REGULATION; VENTRICULAR MYOCYTES; SKELETAL-MUSCLE; PHORBOL ESTERS; RAT-HEART; PHOSPHORYLATION; HYPERTROPHY;
Keywords:
hypertrophy; signal transduction; myofilaments; conditional transgenic; protein kinase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Solaro, RJ Univ Illinois, Coll Med, Dept Physiol & Biophys M C 901, 835 S Wolcott Ave, Chicago, IL 60612 USA Univ Illinois 835 S Wolcott Ave Chicago IL USA 60612 60612 USA
Citazione:
L. Huang et al., "Increased contractility and altered Ca2+ transients of mouse heart myocytes conditionally expressing PKC beta", AM J P-CELL, 280(5), 2001, pp. C1114-C1120

Abstract

Activation of protein kinase C (PKC) in heart muscle signals hypertrophy and may also directly affect contractile function. We tested this idea usinga transgenic (TG) mouse model in which conditionally expressed PKC beta was turned on at 10 wk of age and remained on for either 6 or 10 mo. Comparedwith controls, TG cardiac myocytes demonstrated an increase in the peak amplitude of the Ca2+ transient, an increase in the extent and rate of shortening, and an increase in the rate of relengthening at both 6 and 10 mo of age. Phospholamban phosphorylation and Ca2+-uptake rates of sarcoplasmic reticulum vesicles were the same in TG and control heart preparations. At 10 mo, TG skinned fiber bundles demonstrated the same sensitivity to Ca2+ as controls, but maximum tension was depressed and there was increased myofilament protein phosphorylation. Our results differ from studies in which PKC beta was constitutively overexpressed in the heart and in studies that reported a depression of myocyte contraction with no change in the Ca2+ transient.

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Documento generato il 03/04/20 alle ore 04:07:03