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Titolo:
Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine
Autore:
Vlahou, A; Schellhamrner, PF; Mendrinos, S; Patel, K; Kondylis, FI; Gong, L; Nasim, S; Wright, GL;
Indirizzi:
Eastern Virginia Med Sch, Dept Microbiol & Mol Cell Biol, Norfolk, VA 23507 USA Eastern Virginia Med Sch Norfolk VA USA 23507 Biol, Norfolk, VA 23507 USA Eastern Virginia Med Sch, Dept Urol, Norfolk, VA 23507 USA Eastern Virginia Med Sch Norfolk VA USA 23507 Urol, Norfolk, VA 23507 USA Eastern Virginia Med Sch, Dept Pathol, Norfolk, VA 23507 USA Eastern Virginia Med Sch Norfolk VA USA 23507 thol, Norfolk, VA 23507 USA Virginia Prostate Ctr, Norfolk, VA USA Virginia Prostate Ctr Norfolk VA USA ginia Prostate Ctr, Norfolk, VA USA
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 4, volume: 158, anno: 2001,
pagine: 1491 - 1502
SICI:
0002-9440(200104)158:4<1491:DOANPA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULE E-CADHERIN; MASS-SPECTROMETRY; PROTEIN BIOCHIP; ORAL CARCINOMAS; PROSTATE-CANCER; VOIDED URINE; DEFENSINS; MARKERS; TUMOR; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Vlahou, A Eastern Virginia Med Sch, Dept Microbiol & Mol Cell Biol, 700 W Olney Rd, Norfolk, VA 23507 USA Eastern Virginia Med Sch 700 W Olney Rd Norfolk VA USA 23507 USA
Citazione:
A. Vlahou et al., "Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine", AM J PATH, 158(4), 2001, pp. 1491-1502

Abstract

Development of noninvasive methods for the diagnosis of transitional cell carcinoma (TCC) of the bladder remains a challenge. A ProteinChip technology (surface enhanced laser desorption/ionization time of flight mass spectrometry) has recently been developed to facilitate protein profiling of biological mixtures. This report describes an exploratory study of this technology as a TCC diagnostic tool. Ninety-four urine samples from patients with TCC, patients with other urogenital diseases, and healthy donors were analyzed. Multiple protein changes were reproducibly detected in the TCC group, including five potential novel TCC biomarkers and seven protein clusters (mass range, 3.3 to 133 kd). One of the TCC biomarkers (3.4 kd) was also detected in bladder cancer cells procured from bladder barbotage and was identified as defensin. The TCC detection rates provided by the individual markersranged from 43 to 70% and specificities from 70 to 86%, Combination of theprotein biomarkers and clusters, increased significantly the sensitivity for detecting TCC to 87% with a specificity of 66%, Interestingly, this combinatorial approach provided sensitivity of 78% for detecting low-grade TCC compared to only 33% of voided urine or bladder-washing cytology, Collectively these results support the potential of this proteomic approach for the development of a highly sensitive urinary TCC diagnostic test.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:04:18