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Titolo:
Molecular determinants of ovarian cancer plasticity
Autore:
Sood, AK; Seftor, EA; Fletcher, MS; Gardner, LMG; Heidger, PM; Buller, RE; Seftor, REB; Hendrix, MJC;
Indirizzi:
Univ Iowa Hosp & Clin, Dept Obstet & Gynecol, Div Gynecol Oncol, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin Iowa City IA USA 52242 col, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin, Dept Anat & Cell Biol, Iowa City, IA 52242 USA UnivIowa Hosp & Clin Iowa City IA USA 52242 iol, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin, Dept Pathol, Iowa City, IA 52242 USA Univ Iowa Hosp& Clin Iowa City IA USA 52242 hol, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin, Holden Comprehens Canc Ctr, Iowa City, IA 52242 USAUniv Iowa Hosp & Clin Iowa City IA USA 52242 Ctr, Iowa City, IA 52242 USA
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 4, volume: 158, anno: 2001,
pagine: 1279 - 1288
SICI:
0002-9440(200104)158:4<1279:MDOOCP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; TUMOR ANGIOGENESIS; HUMAN CYTOTROPHOBLASTS; VASCULOGENIC MIMICRY; THROMBIN RECEPTOR; ENDOVASCULAR INVASION; CARCINOMA; CELLS; MELANOMA; HETEROGENEITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Sood, AK Univ Iowa Hosp & Clin, Dept Obstet & Gynecol, Div Gynecol Oncol, 4630 JCP,200 Hawkins Dr, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin 4630JCP,200 Hawkins Dr Iowa City IA USA 52242
Citazione:
A.K. Sood et al., "Molecular determinants of ovarian cancer plasticity", AM J PATH, 158(4), 2001, pp. 1279-1288

Abstract

During development, the formation and remodeling of primary vascular networks occurs by vasculogenesis and angiogenesis. Recently, the term "vasculo-genic mimicry" has been used by our laboratory and collaborators to reflectthe embryonic-like ability of aggressive, but not nonaggressive, melanoma tumor cells to form a pattern of matrix-rich networks (containing channels)surrounding spheroids of tumor cells in three-dimensional culture, concomitant with their expression of vascular cell markers. Ovarian cancer is usually diagnosed as advanced stage disease in most patients when widespread metastases have already been established within the peritoneal cavity, in this study, we explored whether invasive ovarian carcinoma cells could engage in molecular vasculogenic mimicry reflected by their plasticity, compared with their normal cell counterparts. The data revealed that the invasive ovarian cancer cells, but not normal ovarian surface epithelial cells, formed patterned networks containing solid and hollow matrix channels when grown in three-dimensional cultures containing Matrigel or type I collagen, in theabsence of endothelial cells or fibroblasts, Immunohistochemical analysis showed that matrix metalloproteinases (MMP)-1, -2, and -9, and MT1-MMP werediscretely localized to these networks, and the formation of the networks was inhibited by treatment with MMP inhibitors, Furthermore, the RNase protection assay revealed the expression of multiple vascular cell-associated markers by the Invasive ovarian cancer cells. In patient tumor sections fromhigh-stage, high-grade ovarian cancers, 7 to 10% of channels containing red blood cells were lined by tumor cells. By comparison, all vascular areas in benign tumors and low-stage cancers were endothelial lined. These results may offer new insights and molecular markers for consideration in ovariancancer diagnosis and treatment strategies based on molecular vascular mimicry by aggressive tumor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:06:36