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Titolo:
Efficacy of a hepatitis C virus core antigen enzyme-linked immunosorbent assay for the identification of 'window-phase' blood donations
Autore:
Lee, SR; Peterson, J; Niven, P; Bahl, C; Page, E; DeLeys, R; Giordano-Schmidt, D; Baggett, D; Green, G;
Indirizzi:
Ortho Clin Diagnost, AIDS Hepatitis Assay Dev, Raritan, NJ 08869 USA OrthoClin Diagnost Raritan NJ USA 08869 Assay Dev, Raritan, NJ 08869 USA
Titolo Testata:
VOX SANGUINIS
fascicolo: 1, volume: 80, anno: 2001,
pagine: 19 - 23
SICI:
0042-9007(2001)80:1<19:EOAHCV>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTIFICATION; IMMUNOASSAY; TRANSFUSION; INFECTION; RISK;
Keywords:
blood donors; enzyme-linked immunosorbent assay (ELISA); HCV antigen; hepatitis C virus (HCV); window phase; viral RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, SR Ortho Clin Diagnost, AIDS Hepatitis Assay Dev, Raritan, NJ 08869 USA Ortho Clin Diagnost Raritan NJ USA 08869 v, Raritan, NJ 08869 USA
Citazione:
S.R. Lee et al., "Efficacy of a hepatitis C virus core antigen enzyme-linked immunosorbent assay for the identification of 'window-phase' blood donations", VOX SANGUIN, 80(1), 2001, pp. 19-23

Abstract

Background and Objectives Recent studies have suggested that potentially infectious donations provided during the antibody-negative 'window' phase ofhepatitis C virus (HCV) infection may be identified by testing for viral RNA or HCV core protein. We therefore evaluated the performance of an HCV antigen enzyme-linked immunosorbent assay (ELISA) for identification of window-phase donations and for prospective screening of blood donors. Materials and Methods One-hundred and twenty-eight archived plasma donations containing HCV RNA, but lacking antibody to HCV (anti-HCV), were tested by using the HCV antigen ELISA, together with 9951 freshly collected serum and plasma specimens from blood donors. Results HCV core antigen was detected in 94% (120/128) of window-phase plasma donations. Overall specificity in freshly collected blood donor specimens was 99.74%. Two putative window-phase donations containing HCV core protein and viral RNA were identified from paid plasma donors by prospective testing with the HCV antigen ELISA. Conclusion These results indicate that an HCV antigen ELISA can identify almost all (94%) of viraemic donations given during the seronegative window phase of infection. The performance of the HCV antigen ELISA appears to be suitable for large-scale screening of blood donations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:31:18