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Titolo:
Real-time analysis of the interaction of platelets with immobilized thrombospondin under flow conditions
Autore:
Onitsuka, I; Jung, SM; Ikeda, H; Imaizumi, T; Moroi, M;
Indirizzi:
Kurume Univ, Inst Life Sci, Dept Prot Biochem, Fukuoka 8390861, Japan Kurume Univ Fukuoka Japan 8390861 t Prot Biochem, Fukuoka 8390861, Japan Kurume Univ, Dept Internal Med 3, Fac Med, Fukuoka 8300011, Japan Kurume Univ Fukuoka Japan 8300011 Med 3, Fac Med, Fukuoka 8300011, Japan
Titolo Testata:
THROMBOSIS RESEARCH
fascicolo: 6, volume: 101, anno: 2001,
pagine: 455 - 465
SICI:
0049-3848(20010315)101:6<455:RAOTIO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
VON-WILLEBRAND-FACTOR; COLLAGEN TYPE-I; INTEGRIN-ASSOCIATED PROTEIN; HUMAN-BLOOD PLATELETS; MURAL THROMBOGENESIS; VONWILLEBRAND-FACTOR; THROMBUS FORMATION; COATED SURFACE; ADHESION; BINDING;
Keywords:
platelet; thrombosyondin; flow adhesion; vWF (von Willebrand factor); glycoprotein Ib;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Moroi, M Kurume Univ, Inst Life Sci, Dept Prot Biochem, 2432-3 Aikawa Machi, Fukuoka 8390861, Japan Kurume Univ 2432-3 Aikawa Machi Fukuoka Japan 8390861 861, Japan
Citazione:
I. Onitsuka et al., "Real-time analysis of the interaction of platelets with immobilized thrombospondin under flow conditions", THROMB RES, 101(6), 2001, pp. 455-465

Abstract

The platelet granule protein (TS) is extracellularly secreted upon platelet activation and then binds to the platelet surface where it can interact with various adhesive proteins. Here, we have analyzed platelet interactionswith a TS-coated surface under flow conditions, a model for platelet adhesion onto surface-bound TS under physiological conditions. Platelets exhibited temporary, very short-time adhesion on the TS surface, but no firm adhesion. This adhesion was inhibited by NNKY5-5 (anti-glycoprotein (GP) Ib antibody) and AJvW-2 (anti-von Willebrand factor (VWF)), indicating that both platelet GP Ib and plasma VWF contribute to this interaction. Antibodies against platelet collagen receptor integrin alpha (2)beta (1) had no significant effect. These results suggested that binding of VWF to TS is the first step in platelet interaction with the TS surface. By surface plasmon resonance spectroscopy, a dissociation constant (Kd) Of 3.97 x 10(-7) M was obtained for the binding reaction between immobilized TS and VWF. These results suggest the following model for platelet interaction with the TS surface under flow: plasma vWF first binds to the immobilized TS and then platelets interact with the TS-bound vWF. A low density of bound VWF would account for the observed weak interaction between TS and platelets under flow. (C) 2001Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:31:00