Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos
Autore:
Gutierrez-Adan, A; Lonergan, P; Rizos, D; Ward, FA; Boland, MP; Pintado, B; de la Fuente, J;
Indirizzi:
INIA, Dept Reprod Anim & Conservac Recursos Zoogenet, Madrid 28040, Spain INIA Madrid Spain 28040 Conservac Recursos Zoogenet, Madrid 28040, Spain Univ Coll Dublin, Dept Anim Sci & Prod, Dublin, Ireland Univ Coll Dublin Dublin Ireland , Dept Anim Sci & Prod, Dublin, Ireland
Titolo Testata:
THERIOGENOLOGY
fascicolo: 5, volume: 55, anno: 2001,
pagine: 1117 - 1126
SICI:
0093-691X(20010315)55:5<1117:EOTIVC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; CONDITIONED MEDIUM; CLEAVAGE RATES; OVINE EMBRYOS; IVF EMBRYOS; OVIDUCT; INVITRO; SERUM; INSEMINATION;
Keywords:
bovine embryos; IVC; coculture; FCS; sex ratio;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Gutierrez-Adan, A INIA, Dept Reprod Anim & Conservac Recursos Zoogenet, Ctra Coruna Km 5-9, Madrid 28040, Spain INIA Ctra Coruna Km 5-9 Madrid Spain28040 28040, Spain
Citazione:
A. Gutierrez-Adan et al., "Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos", THERIOGENOL, 55(5), 2001, pp. 1117-1126

Abstract

Bovine blastocysts were produced using 6 different systems: 5 commonly used in vitro culture systems (synthetic oviduct fluid medium - SOF- without fetal calf serum, SOF supplemented with 10% serum for the entire culture period, SOF supplemented with 10% serum from Day 4 of culture, M199 coculture with bovine oviduct epithelial cells, M199 coculture with granulosa cell monolayer) and 1 in vivo culture system involving collection of blastocysts from superovulated bovine donors at Day 7. Zygotes obtained from IVM/IVF were assigned randomly to 1 of the 5 systems tested and were cultured for 9 d (Day 0= day of insemination). Cleavage, development to the blastocyst stageand blastocyst sex ratio were assessed in all treatments. In addition, theeffect of the IVC system on the kinetics of blastocyst development and sexratio was assessed on Days 6, 7, 8, and 9. The presence of fetal calf serum in SOF not only resulted in faster development (19.1% of blastocysts in SOF supplemented with serum vs 7.1% in absence of serum at Day 6; P < 0.05) and increased blastocyst production (47.5% of blastocysts in SOF supplemented with serum vs 34.4% in absence of serum; P < 0.05) but it also enhanced overall male survival. The coculture systems produced fewer blastocysts than culture in SOF (27.6 to 28.3% in coculture vs 47.5% in SOF supplemented with serum; P < 0.05), but similar to SOF without fetal calf serum, they hadno effect on blastocyst sex ratio. <(c)> 2001 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:20:47