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Titolo:
Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis
Autore:
Kaneko, M; Tomita, T; Nakase, T; Ohsawa, Y; Seki, H; Takeuchi, E; Takano, H; Shi, K; Takahi, K; Kominami, E; Uchiyama, Y; Yoshikawa, H; Ochi, T;
Indirizzi:
Osaka Univ, Sch Med, Dept Orthopaed Surg, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ed Surg, Suita, Osaka 5650871, Japan Osaka Univ, Sch Med, Dept Cell Biol & Anat 1, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Anat 1, Suita, Osaka 5650871, Japan Juntendo Univ, Sch Med, Dept Biochem, Tokyo 113, Japan Juntendo Univ Tokyo Japan 113 v, Sch Med, Dept Biochem, Tokyo 113, Japan
Titolo Testata:
RHEUMATOLOGY
fascicolo: 3, volume: 40, anno: 2001,
pagine: 247 - 255
SICI:
1462-0324(200103)40:3<247:EOPAIC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; COLLAGEN-INDUCED ARTHRITIS; IN-SITU HYBRIDIZATION; CATHEPSIN-B; MARROW ADJACENT; IV COLLAGENASE; MESSENGER-RNA; STROMAL CELLS; FACTOR-ALPHA; INTERLEUKIN-1;
Keywords:
rheumatoid arthritis; cathepsins; matrix metalloproteinase 9 (MMP-9); inflammatory cytokines; joint destruction; immunohistochemistry; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Ochi, T Osaka Univ, Sch Med, Dept Orthopaed Surg, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 5650871, Japan
Citazione:
M. Kaneko et al., "Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis", RHEUMATOLOG, 40(3), 2001, pp. 247-255

Abstract

Objective. We previously described abnormalities in the bone marrow of patients with rheumatoid arthritis (RA), but were able to shed little light onthe pathogenic roles of inflammatory cytokines and proteinases in joint destruction in the subchondral region in RA. This is the first report to describe the co-localization of cytokines and proteinases in this area. Methods. Decalcified paraffin-embedded sections from 10 patients with RA and five patients with osteoarthritis (OA) were examined for the immunolocalization of cathepsins B, K and L and the localization of messenger RNAs forinterleukin 1 beta (IL-1 beta), tumour necrosis factor alpha (TNF-alpha) and matrix metalloproteinase 9 (MMP-9). The cells were double-stained with anti-CD68 or anti-prolyl 4-hydroxylase (PH) antibody. Results. An immunohistochemical study confirmed the expression of cathepsins B and L by CD68-positive mononuclear cells at the sites of significant cartilage and bone erosion from the subchondral region in all RA specimens. Osteoclast-like cells showed intense staining for cathepsin K and MMP-9. Osteoblast-like cells strongly expressed MMP-9. Analysis of serial sections revealed that expression of the IL-1 beta and TNF-alpha genes occurred near that of the cathepsins and MMP-9 in the subchondral region. Conclusion. We conclude that inflammatory cytokines and tissue-damaging proteinases play important roles in joint destruction in the subchondral region in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:12:09